Hematological parameters are useful indicators for assessing the physiological conditions of Sturgeon in response to stress, pollutants and physiological and ecological changes. The aim of this study was to investigate the effects of nitrosomonase and nitrobacter in removing ammonia and on blood and serum indices of Huso huso .Four treatments were designed with densities of bacteria (0, 10¹, 10³, 10⁵) in 100 ml with three replications were performed using 120 sturgeon fish with an average weight of 200 ± 800 g and a length of 55±2 cm in 12 fiberglass tanks (4000 liters per each 10 fish) for 12 weeks and two weeks of initial compatibility. Based on the results, the highest amount of ammonia was observed in treatment 1 (control) with significant difference (p≤0.05) with treatment 3 and no significant difference with other treatments. Ammonia levels were not significantly different (p≥0.05) between the hours measured in each treatment. Among the blood parameters, only neutrophil was significantly different in treatments (p≤0.05). Serum blood parameters such as aspartate aminotransferase (AST), alkaline transferase (ALP), keratin phosphatase (CP), total protein and alanine aminotransferase (ALT) were not significantly different (p≥0.05). Lactic dehydrogenase (LDH) had a significant difference with other treatments (p<0.05). Nitrosommunos and nitrobacter had a positive effect to remove ammonia from the water of the culture environment and had no negative effect on the physiological conditions of sturgeon (huso huso) blood parameters.