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:: Volume 28, Issue 2 (7-2019) ::
isfj 2019, 28(2): 95-104 Back to browse issues page
The Primary cell culture from caudal fin tissue of Caspian Kutum (Rutilus frisii kutum)
M. Hadifar1 , S. Haghighi Karsidani * 1, M. Ghasemi2
1- Department of Fisheries, Bandar Anzali Branch, Islamic Azad University, Bandar Anzali, Iran
2- Inland Water Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Anzali, Iran
Abstract:   (2896 Views)
In the present study, the primary cell lines were derived from caudal fin explants of the Caspian Kutum (Rutilus frisii kutum), using two treatments (tissue culturing without enzyme and after trypsin treatment). For this purpose, pieces of caudal fin tissue (1 millimeter cubic) were isolated from 10 healthy young fish with an average weight of 12±1 g and a mean length of 11±1cm in sterile condition then were explanted in L-15 medium (Leibovitz L-15) with fetal bovine serum (10 and 20%) and penicillin-streptomycyne at pH 6.8 and 7.2 and kept at 21 and 24 °C. From the second day, a mixture of various cell types was observed and attached to the cell culture flask, but the uniform cell layer consist of cells with heterogeneous epithelial morphology and fibroblast-like were appeared after 12 to 14 days. Subculture was performed using trypsin enzyme. In subcultures, a single cell layer consists of cells with homogeneous epithelial morphology was formed. So far, these cells have been passaged for 21 times. Results indicate the possibility of producing a permanent cell line from the fin tissue of Rutilus frisii kutum.
Keywords: Tissue culture, Caudal fin, Rutilus frisii kutum
Full-Text [PDF 641 kb]   (1412 Downloads)    
Type of Study: Research | Subject: بهداشت بيماريها
Received: 2018/12/9 | Accepted: 2019/05/22 | Published: 2019/07/15
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Hadifar M, Haghighi Karsidani S, Ghasemi M. The Primary cell culture from caudal fin tissue of Caspian Kutum (Rutilus frisii kutum). isfj 2019; 28 (2) :95-104
URL: http://isfj.ir/article-1-2138-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 2 (7-2019) Back to browse issues page

با کسب مجوز از دفتر کمیسیون بررسی نشریات علمی وزارت علوم، تحقیات و فنآوری مجله علمی شیلات بصورت آنلاین می باشد و تعداد محدودی هم به چاپ می رساند. شماره شاپای جدید آن ISSN:2322-5998 است

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