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Survey of pollution in areas of Anzali wetland to Yersinia enterocolitica and Listeria monocytogenes by microbial culture and PCR and their relationship to the pH and electrical conductivity of water
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Mohamad hadi Abolhasani   , Zahra Talebi * 1, Maryam Payami Khomeran |
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Abstract: (1800 Views) |
Anzali wetland is the most important intermediate ecosystem in the Caspian sea catchment area and plays an important role in refining the inputs of rivers in this area and acts as a natural filter. In this study, the overall microbial load and bacteria of Yersinia enterocolitica and Listeria monocytogenes and its relationship with pH and EC of water were investigated. Surface layer sampling was performed from autumn 2018 to summer 2019 using standard water sampling methods. The highest logarithmic mean of the total bacterial count was 5.513/ ml at Ali Abad station in summer and the lowest logarithmic mean of the total bacterial count was 3.914/ ml at Abknar station in autumn and above standard. The rate of contamination of different wetlands to Yersinia enterocolitica and Listeria monocytogenes using specific media was 41% and 50% respectively and in the PCR method with specific primer pairs, were 35.7% and 42.8% respectively. The highest pH in Sangachin station was 8.86 and the highest EC was 1050μs / cm in Talebabad station. Duncan test showed a significant difference at most stations with the percentage of specific cultures, pH and EC factors and the logarithmic mean of total bacterial count (p<0.05). Also, using Duncan's test in the PCR method, Listeria monocytogenes in autumn were statistically significantly different from other seasons (p<0.05) and Yersinia enterocolitica was statistically significant in autumn and summer (p<0.05). Elevated water temperatures, urban population growth around wetlands, and sewage entry are major causes of increased the level of pollution in Anzali wetland and its transmission through the food cycle and indicates the risk of pathogenicity.
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| Keywords: Anzali Wetland, Yersinia enterocolitica, Listeria monocytogenes, Pollution |
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Full-Text [PDF 586 kb]
(502 Downloads)
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Type of Study: Research |
Subject:
آلودگي محيطهاي آبي
Received: 2020/01/4 | Accepted: 2020/08/31 | Published: 2020/08/31
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