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:: Volume 21, Issue 2 (7-2012) ::
isfj 2012, 21(2): 99-114 Back to browse issues page
Effect of LHRHA2 and carp pituitary extract hormone on some spermatozoa fertility and quality indices in Barbus sharpeyi
M.R Kalbassi * 1, R. Lorestani , J. Ghafleh Marammazi
Abstract:   (10248 Views)
Effects of LHRHA2 hormone in 2.5, 5 and 10μg/kg doses and anti-dopamine metoclopramide on Barbus sharpeyi spermiation, spermatozoa quality and fertility indices, chemical (Na+, K+ and Ca+2) and biochemical component (Alkaline phosphates, Glucose and Triglyceride) of seminal plasma and its osmotic pressure were assessed in comparison to Carp pituitary extract treatment. Results indicated that the highest incubation survival, hatching rate, sperm volume, duration of sperm motility as well as the lowest larval deformity were achieved by injection of LHRHA2 (10μg/kg +2.5mg/kg metoclopramide) and it was significantly different to others groups. Application of above mentioned treatment had the highest osmotic pressure with about (Mean ±SD) 324±7.31mOsmol kg-1 among other groups. Also, the highest level of Na+ was assessed by injection of LHRHA2 (10μg/kg +2.5mg/kg metoclopramide). Result showed that injection of Carp pituitary extract caused the highest spermatocrit, K+ value and larval deformity in comparison to other treatments. In conclusion, optimum sperm quality can be produced by injection of LHRHA2 (10μg/kg + 2.5mg/kg metoclopramide) in males of B. sharpeyi at 8 hour following hormonal stimulation. Also, above mentioned treatment caused increasing of incubation survival and decrease of larval deformity in B. sharpeyi.
Keywords: Pituitary gland, Sperm, Plasma, Barbus sharpeyi, Iran
Full-Text [PDF 338 kb]   (3572 Downloads)    
Type of Study: Research | Subject: فيزيولوژي آبزيان
Received: 2013/05/13 | Accepted: 2015/06/17 | Published: 2015/06/17
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Kalbassi M, Lorestani R, Ghafleh Marammazi J. Effect of LHRHA2 and carp pituitary extract hormone on some spermatozoa fertility and quality indices in Barbus sharpeyi . isfj 2012; 21 (2) :99-114
URL: http://isfj.ir/article-1-712-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 2 (7-2012) Back to browse issues page

با کسب مجوز از دفتر کمیسیون بررسی نشریات علمی وزارت علوم، تحقیات و فنآوری مجله علمی شیلات بصورت آنلاین می باشد و تعداد محدودی هم به چاپ می رساند. شماره شاپای جدید آن ISSN:2322-5998 است

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