RT - Journal Article T1 - Karyology study of Spirlin (Alburnoides bipunctatus) in Zabol region JF - ISFJ YR - 2013 JO - ISFJ VO - 22 IS - 2 UR - http://isfj.ir/article-1-1012-fa.html SP - 137 EP - 146 K1 - Zabol K1 - Karyotype K1 - Spirlin K1 - Alburnoides bipunctatus AB - The chromosomal spread and karyotype of Spirlin (Alburnoides bipunctatus) from Zabol region were identified using tissue squashing techniques with injection of 0.5ml/100g body weight of 0.01% Colchicines solution in fish fingerlings. Kidney and gill tissues were extracted and chopped in KCl 0.075M for 30 min and fixed in Carnoy solution in 3 stages. The chromosomal spreads were stained in 20% Gimsa for 20 min. From 34 chromosomal spread counts, the results showed diploid chromosome number of this species 2n=50. Karyotype composed of 9 metacentric, 14 submetacentric and 2 acrocentric or subtelocentric chromosome pairs, and the number of chromosome arms (NF) was determined as NF=96 LA eng UL http://isfj.ir/article-1-1012-fa.html M3 10.22092/ISFJ.2017.110126 ER -