fa مقاله علمی – پژوهشی: مقایسه فعالیت آنتی‌اکسیدانی و بتا کاروتن استخراج شده از سه گونه جلبک‌های بومی دریای عمان (Ulva lactuca، Sargassum ilicifolium و Nizimuddinia zanardini) بيوتكنولوژي و فرآوري هاي شيلاتي بيوتكنولوژي و فرآوري هاي شيلاتي پژوهشي Research <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">در این مطالعه خواص فارماکولوژیک سه گونه از ماکروجلبک‌‌های بومی سواحل چابهار (دریای عمان) شامل: </span></span></span><a name="OLE_LINK8"></a><a name="OLE_LINK7"><em><span dir="LTR"><span style="color:black;">Ulva lactuca</span></span></em></a><a name="OLE_LINK6"></a><a name="OLE_LINK5"></a><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">، </span></span></span><em><span dir="LTR"><span style="color:black;">Sargassum ilicifolium</span></span></em> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">و </span></span></span><em><span dir="LTR"><span style="color:black;">Nizimuddinia zanardini</span></span></em> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">بررسی شد</span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">.</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">عصاره</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">متانولی</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">سه</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">گونه</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">جلبکی سبز،</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">قرمز</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">قهوه</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">ای</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">تهیه</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">گردید</span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">. اندازه‌گیری میزان بتا-کاروتن، فنلی کل و قدرت مهارکنندگی رادیکال آزاد </span></span></span><span dir="LTR"><span style="color:black;">DPPH</span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">بر اساس روش‌های معمول آزمایشگاهی انجام شد. بر اساس نتایج</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">بدست آمده</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">خواص</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">آنتی‌اکسیدانی</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">متفاوتی</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">&nbsp;بین جلبک‌‌ها مشاهده شد. </span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">نتایج احتمالاً</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">متناسب</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">با محتوای</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">ترکیبات</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">آنتی‌اکسیدانی</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">&nbsp;جلبک‌‌ها</span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;"> بود. مقدار شاخص های مذکور&nbsp; در </span></span></span><em><span dir="LTR"><span style="color:black;">U. lactuca</span></span></em><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">، </span></span></span><em><span dir="LTR"><span style="color:black;">S. ilicifolium</span></span></em><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;"> و </span></span></span><em><span dir="LTR"><span style="color:black;">N. zanardini</span></span></em><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;"> &nbsp;بترتیب برای بتاکاروتن 56/0، 048/0 و 041/0 میلی‌گرم در 100 گرم وزن خشک، فنول کل 206/0، 396/0 و 133/0 میلی‌گرم</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">اسید</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">گالیک</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">بر</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">گرم</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">وزن خشک و قدرت مهارکنندگی رادیکال آزاد</span></span></span> <span dir="LTR"><span style="color:black;">DPPH </span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">&nbsp;7/45-4/60، 6/51-8/61 و 6/9-28 درصد اندازه‌گیری شد. بر این اساس </span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">بیشترین مقدار بتا کاروتن در عصاره </span></span></span><em><span dir="LTR"><span style="color:black;">U. lactuca</span></span></em> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">و بیشترین مقدار فنل و قدرت مهارکنندگی رادیکال آزاد </span></span></span><span dir="LTR"><span style="color:black;">DPPH</span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;"> در عصاره </span></span></span><em><span dir="LTR"><span style="color:black;">S. ilicifolium</span></span></em> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">ثبت شد (05/0</span></span></span><span dir="LTR"><span style="color:black;">p<</span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">).</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">در مجموع نتایج این تحقیق نشان داد </span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">هر</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">سه</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">جلبک </span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">از </span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">قابلیت بالایی</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">برای </span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">استفاده</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">به‌عنوان</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">یک</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">ماده</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">اولیه در صنایع غذایی و</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">دارویی</span></span></span> <span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">برخوردار هستند</span></span></span><span style="color:black;"><span style="font-family:B Compset;"><span style="font-size:12.0pt;">.</span></span></span> <a name="OLE_LINK9">In this study, pharmacological properties of three native macro algae which collected from the shores of the Chabahar Gulf (the Omean Sea) including: <em>Ulva lactuca</em>, <em>Sargassum ilicifolium </em>and <em>Nizimuddinia zanardini</em> were evaluated.</a> The methanol extracts of three species of green, brown and red algae were. The concentration of &beta;-carotenoid, phenolic Compound and antioxidant activities (AOA) was analyzed by routine methods. Based on the results, different antioxidant properties were observed among algae. These results probably was associated with antioxidant compounds of algae. In the species of <em>U. lactuca, S. ilicifolium</em> and <em>N. zanardini</em> the amount of &beta;-carotenoid was measured 0.56, 0.048 and 0.041 mg/100DW, total phenol was 0.206, 0.396 and 0.133 mg GA/g DW and inhibitory activity of free radicals (DPPH) was in the range of 45.7-60.4%, 51.6-61.8% and 9.6-28%, respectively (p<0.05). Based on obtaining results, the highest level of &beta;- carotenoid was observed in <em>the U. lactuca</em> extract and the highest level of total phenol and i<a name="OLE_LINK37"></a><a name="OLE_LINK36">nhibitory activity of free radicals</a> (DPPH) <a name="OLE_LINK4"></a><a name="OLE_LINK3">was observed in </a><em>S. ilicifolium</em> extract. Overall, the results of this study indicated that all three species of algae have usability as a primary substance in food and drug industry. جلبک, سیستان و بلوچستان, دریای عمان, خواص آنتی‌اکسیدانی, بتا کاروتن Algae, Sistan and Balochestan, Oman Sea, Antioxidant properties, β-carotene 53 63 http://isfj.ir/browse.php?a_code=A-10-2524-1&slc_lang=fa&sid=1 Eidmohammad Sarani yaztapeh عیدمحمد سارانی یازتپه mohammad.sarani49@gmail.com 100319475328460031143 100319475328460031143 No Zahedan Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد زاهدان Mohammadreza Hosseini_tabatabaei سید محمد رضا حسینی طباطبایی Dr_hosseini_tabatabaei@yahoo.com 100319475328460031144 100319475328460031144 Yes Zahedan Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد زاهدان Ali Mahdi Abkener علی مهدی آبکنار aliabkenar@gamil.com 100319475328460031145 100319475328460031145 No Bandar Abbas Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد بندر عباس