fa مقاله علمی – پژوهشی:‌ ارزیابی فعالیت کمپلمان سرم تمساح مردابی (Crocodylus palustris) بهداشت و بيماريهاي آبزيان بهداشت بيماريها پژوهشي Research <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">سیستم کملپمان به عنوان یکی از اجزاء مهم سیستم ایمنی ذاتی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">در کروکودیل‌ها کمتر شناخته شده است. </span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">ارزیابی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">فعالیت کمپلمان سرم</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> به عنوان</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> یک</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> ابزار با ارزش برای تشخیص </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">وضعیت سلامتی</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> کروکودیل‌ها </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">محسوب می‌شود. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">در این ﭘژوهش، پاسخ ایمنی ذاتی تمساح مردابی یا پوزه‌کوتاه </span></span>(<i>Crocodylus palustris</i>)<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">، بزرگترین خزنده بومی ایران، با استفاده از همولیز گلبول‌های قرمز خرگوش و</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">کروکودیل</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> مورد بررسی قرار گرفت. در</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">این مطالعه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">تأثیر غلظت، حجم و دما</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> بر</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> فعالیت مسیر جایگزین کمپلمان </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">تمساح مردابی </span></span>(<i>C. palustris</i>)<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> با استفاده از روش های همولیتیک استاندارد انجام و اندازه گیری میزان همولیز صورت گرفت</span></span>.<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> بر اساس نتایج به‌دست آمده ارتباط معنی‌داری بین غلظت،</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">حجم و دما با </span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">میزان </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">همولیزگلبول‌های قرمز</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">خرگوش</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">وجود داشت (</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">05/0</span></span><i>P</i><<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">).</span></span> <span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">میزان همولیز سرم در </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">غلظت‌ها</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> (25 درصد، 50 درصد و 100 درصد)، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">حجم</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> ها (10، 20 </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">و</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">30 </span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">میکرولیتر)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> و </span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">دماهای مختلف (35-5 درجه </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">سانتی‌گراد</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">) به طور معنی</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">‌</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">داری افزایش یافت</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">(</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">05/0</span></span>P<<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">)</span></span>. <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">نتایج</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> نشان می</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">‌</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">دهد</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">که</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> فعالیت مسیر </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">جایگزین کمپلمان</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">کروکودیل‌</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> ممکن است </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">تحت</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">تأثیر غلظت، حجم و دما</span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> قرار </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">گیرد </span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">که این سبب افزایش </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">پاسخ ایمنی </span></span><span lang="PS-AF" style="font-size:12.0pt"><span b="" compset="" style="font-family:">غیراختصاصی و ایجاد مقاومت </span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">به شیوع بیماری‌ها</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">می‌گردد</span></span> .</span></span></span></span> <span style="font-size:11pt"><span style="line-height:17.0pt"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Little is known about </span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">complement system </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">as a component of innate immunity</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> of the </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">ectothermic vertebrates such as</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> crocodiles. Serum complement is valuable tool in determining the health status of turtles.<b><i> </i></b>In this research effect of concentration, volume and temperature </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">on alternative complement pathway activity</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> of </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Mugger crocodiles (<i>Crocodylus palustris</i>) </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">using standard haemolytic assays</span></span> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">was studied. For this purpose</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">,</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> <i>Crocodylus palustris</i></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> serum complement hemolysis in different concentrations, volumes and temperatures was </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">measured</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.</span></span> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results indicated significant relation between relation measured hemolysis and </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">concentration, volume and temperature (p<0.05). The measure of </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">hemolysis</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> in various concentrations (25%, 50% and 100%), volumes (10, 20, 30 &micro;L) and temperatures (5-35&deg;C) were </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">significantly </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">increased (p<0.05). The results show that alternative complement pathway activity of crocodiles might affect concentration, volume and temperature that it caused increased non-specific immunity and the resistance to the outbreak of diseases. </span></span><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></b></span></span></span></span><br> <br> <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Arial",sans-serif"></span></span></span></span></span></span></span> پاسخ ایمنی, کمپلمان, ایمنی ذاتی, تمساح مردابی Complement, Crocodiles, Non-specific immunity, Serum 63 67 http://isfj.ir/browse.php?a_code=A-10-2783-1&slc_lang=fa&sid=1 Azadeh Yektaseresht آزاده یکتاسرشت azadeh_neisi@shirazu.ac.ir 1754966225 100319475328460036757 Yes Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. گروه ﭘاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران Siyavash Soltanian سیاوش سلطانیان Siyavash.Soltanian@yahoo.com 100319475328460036758 100319475328460036758 No Department of Clinical Sciences, Department, Veterinary School, Shiraz University Shiraz, Iran 2گروه علوم درمانګاهی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران Amin Gholamhosseini امین غلامحسینی Amin.Gholamhosseini@yahoo.com 100319475328460036759 100319475328460036759 No Department of Clinical Sciences, Department, Veterinary School, Shiraz University Shiraz, Iran 2گروه علوم درمانګاهی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران Shirin Safaeian شیرین صفاییان Shirin.Safaeian@yahoo.com 100319475328460036760 100319475328460036760 No 3DVM Graduated, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. 3دانش آموخته دانشکده دامپزشکی دانشگاه شیراز، شیراز، ایران Hesamodin Kordestani حسام الدین کردستانی Hesamodin.Kordestani@yahoo.com 100319475328460036761 100319475328460036761 No 3DVM Graduated, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. 3دانش آموخته دانشکده دامپزشکی دانشگاه شیراز، شیراز، ایران