IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
1
22
FA
sunshineliyan@gmail.com
Y
N
N
10.22092/ISFJ.2017.110082
http://isfj.ir/article-1-794-en.html
http://isfj.ir/article-1-794-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Effect of Zataria multiflora essential oils on rainbow trout (Oncorhynchus mykis) complement component activity and lysozyme
13
23
FA
M.
Soltani
msoltani@ut.ac.ir
Y
T.
Zarifmanesh
N
S.J.
Zorriehzahra
N
10.22092/ISFJ.2017.110083
Influence of dietary administration of Zataria multiflora Bioss was evaluated on complement component activity and lysozyme of rainbow trout (Oncorhynchus mykiss) to determine stimulatory effect of essential oils. Fish weighting 100±10 g were fed with different doses of 10, 20 and 40 ml/kg food for a period of 10 days. Alternative Complement Activity (CH50%) and serum lysozyme activity were measured on days 1, 8, 15 and 29 after the essential oils administration. The obtained results showed that for first and eighth days of sampling, the complement component mean in treatment groups were significant higher than control group, while negligible difference is shown between treatment groups. For fifteenth and twenty ninth days of sampling, no significant difference was seen in the complement component mean among all examined groups. Also, the results of experiment show that for all of days of sampling, essential oils of Zataria multiflora Bioss have no effect on serum lysozyme activity.
Essential Oils of Zataria multiflora, Oncorhynchus mykiss, Fish Immune System
http://isfj.ir/article-1-795-en.html
http://isfj.ir/article-1-795-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Identification, abundance and biomass of benthic communities in south east coasts of the Caspian Sea (Golestan Province)
23
31
FA
S.
Sharbati
Safoora.sharbati@gmail.com
Y
R.
Akrami
N
S.
Yelghi
N
J.
Mirdar
N
Z.
Ahmadi
N
10.22092/ISFJ.2017.110084
The frequency and distribution of benthic species in the south east coast of Caspian Sea (proposed site for cage and pen culture) were studied. Sampling was carried out in 2 water depths (1, 5) meters and 12 stations were sampled in each depth using VanVeen sampler. Totally, 11 taxa were identified: Pyrgulidae,Ampharetidae, Neritidae, Nereidae, Cardidae, Gammaridae, Naididae, Balanidae, Foraminifera, Ostracoda and Cumaceae. The most abundant taxa were Gastropoda (66.36%), Foraminifera (15.66%), Polychaeta (14.09%) and Bivalvia (1.65%) respectively. The maximum and minimum biomass was 164.1 g/m in summer and 6.56 g/m in spring. Depth, substratum, season, season-depth interaction, season- substratum –depth interaction had significant effects on biomass and had no significant effect on abundance.
Macro benthic, Invertebrates, Caspian Sea, Iran
http://isfj.ir/article-1-796-en.html
http://isfj.ir/article-1-796-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Investigation of anesthetizing effect of tobacco (Nicotiana tabacum) aqueous and alcoholic extract on Rainbow trout (Oncorhynchus mykiss)
33
40
FA
D.
Zargham
davoodzargham@hotmail.com
Y
M.
Sharifrohani
N
I.
Falahat Naserabad
N
T.
Bashti
N
10.22092/ISFJ.2017.110085
The main goal of anesthesia in rainbow trout is decrease mobility and reduce stress of fish while breeding, and also facilitate operation such as manipulation to Tagging, Biometry, Sorting, Inspection and Sampling for pathology test. In the present study, Aqueous and alcoholic extract of tobacco plant (Nicotiana tabacum) for Anaesthetizing Rainbow trout (Oncohrynchus mykiss) in 3 weight groups: fry (less than 80 g), growing(about 200 g) and breeding(larger than 1500g) were used. The effective dose of tobacco in treatments 200, 400, 800 and 1600mg/lit in aqueous and 0.7, 1, 1.7 and 3.5mg/lit in alcoholic extract were tested. Totally, we can say that tobacco extract Anaesthetizes the rainbow trout in less than 3 minutes and recovery time is less that 5 minutes. Regarding to anesthetizing time, recovery time and fish relaxing during anesthesia, aqueous extract applies more gentle anesthesia in rainbow trout, and dose 200 to 800 mg/lit of aqueous extract were more successful. Also Regarding to significant correlation between doze and Anesthetics extremity and recovery time (P<0.05), lower dose of tobacco extract can be used to making fish calm for transportation. *Corresponding author
Tobacco, Rainbow trout, Anesthetic
http://isfj.ir/article-1-797-en.html
http://isfj.ir/article-1-797-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Effect of Dietary Protein and Energy Levels on the Growth and Body Composition of Juvenile Orange-spotted Grouper, Epinephelus coioides
41
56
FA
J.
Marammazi
jmarammazi@yahoo.com
Y
E.
Pagheh
N
Z.
Mokhaiier
N
10.22092/ISFJ.2017.110086
To evaluate the protein and energy requirement of orange-spotted grouper, Epinephelus coioides fingerlings, nine experimental diets including 3 levels of crude protein (40%, 50% and 60%) and 3 levels of digestible energy (DE, 14, 16 and 18KJ.g-1), were tested. Triplicate 300 L circular tanks provided with flow-through water system (2L. min-1) and air stone and each stocked with 20 juvenile of grouper fish (16.79±0.4g) were applied to adopt an experimental test for 8-weeks rearing period. The stocked fishes were fed experimental diets to approximate satiation, twice a day. Results showed that the survival rate, hepatosomatic index (HSI) and condition factor (CF) were independent of the dietary treatments, but final weight, weight gain, specific growth rate(SGR), feed efficiency (FE), feed conversion ratio (FCR), protein efficiency ratio (PER) and daily feed intake (DFI) were significantly affected by protein and digestible energy levels (P<0.05). Diet 5 (50%CP and 16KJ.g-1 DE) was the preferred among the tested diets and resulted in the best growth performance, feed and protein efficiency and nutrient utilization among the examined diets. DFI was the lowest for diet with the highest protein (60%) and energy (18KJ.g-1). Apparent net protein utilization (ANPU) varied insignificantly (P>0.05) between the treatments. viscerosomatic index(VSI) increased significantly with the increasing of diet energy level. Body lipid, ash and moisture contents were affected by dietary protein and energy levels. Body lipid content increased with increasing dietary energy levels, but carcass ash and moisture decreased. Body protein content was not affected by dietary energy level, but significantly increased when protein increased from 40% to 50% at same energy levels (P<0.05). The study revealed that fingerlings of orange-spotted grouper, E. coioides showed highest growth performance when fed diet containing 50% crude protein, 16KJ.g-1 digestible energy with the P/DE ratio, of 31.25mg/kJ. *Corresponding author
Epinephelus coioides, protein, energy, growth, body composition
http://isfj.ir/article-1-798-en.html
http://isfj.ir/article-1-798-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Changes in stocks of anchovy kilka, Clupeonella engrauliformis in Iranian coastal zone of the Caspian Sea (1995-2011)
57
66
FA
H.
Fazli
hn.fazli@yahoo.com
Y
A.A
Janbaz
N
F.
Keymaram
N
SH.
Abdolmaleki
N
K.
Khedmatei
N
10.22092/ISFJ.2017.110087
The main objective of the present study was qualitative and quantitative assessment of anchovy stock, Clupeonella engrauliformis in Iranian waters of the Caspian Sea during (1995- 2011). Over this period, total catch of anchovy kilka increased from 32000 tonnes in 2005 to 67000 tonnes in 1999 and decreased sharply to 80 tonnes in 2011. In the years 1995-1999, catch per unit effort (CPUE) was fairly constant (2.92-3.52 tonnes/(Vessels×Nights) which fell to 0.01 tonnes/(Vessels×Nights) in 2011. Total biomass and spawning stock biomass (SSB) had the same trend as CPUE. During 1995-1999, total biomass of anchovy kilka ranged between 158500-185900 tonnes which dropped to 2000 tonnes in 2007. The SSB showed a downwardtrend from 105500 tonnes in 1997 to 1950 tonnes in 2007. In age compositions of the catch, age 3+ was the largest age group. From 2001 to 2003, age 2+ predominated. Finally, number of young specimens declined and age 6+ and 7+ groups were predominated in the last years. Present study indicated that the situation of the anchovy kilka is critical. A coordinated international effort is needed to provide implementation of sustainable management and responsible fishing activities in the whole Caspian Sea. *Corresponding author
Catch, Stock assessment, Clupeonella engrauliformis, Caspian Sea
http://isfj.ir/article-1-799-en.html
http://isfj.ir/article-1-799-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
67
74
FA
Y
N
10.22092/ISFJ.2017.110088
http://isfj.ir/article-1-800-en.html
http://isfj.ir/article-1-800-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Determination of growth parameters of the king soldier bream (Argyrops spinifer), using the backcalculation method and otolith reading data in coastal waters of Bushehr Province, Persian Gulf
75
84
FA
M.
Ghanbarzadeh
Y
N.
Mahboobi Soofiani
N
Y.
Keivany
N
S.
Asadollah
N
S.A.
Taghavi Motlagh
N
10.22092/ISFJ.2017.110089
Age and growth of the king soldier bream, Argyrops spinifer (Forsskal 1775) in the Persian Gulf (Bushehr coasts) were investigated. A total of 622 samples were obtained from June 2010 to May 2011. Total length (TL) of fish ranged from 13.5 to 64.2cm (females) and 15 to 61.2cm (males). Male and female fish age ranged between 2+ to 25+ and 2+ to 21+ years, respectively. Values of the von Bertalanffy growth function were calculated in females, males and total population by using the backcalculation method and otolith reading data. The values of these parameters according to otolith reading data were K=0.061, L∞=82.10cm (TL), t0=- 2.39 years for females and K=0.082, L∞=67.90cm (TL), t0= - 2.88 for males and K=0.065, L∞=75.50cm (TL), t0=-3.21 for total population and according to backcalculation method were K=0.088, L∞=76.37cm (TL), t0=-0.329 years for females and K=0.101, L∞=65.71cm (TL), t0=-0.168 for males and K=0.094, L∞=74.55cm (TL), t0=-0.191 for total population. The results of the study provide the direction for the future management models for the continued sustainable exploitation of this species in the Persian Gulf region.
Stock assessment, Bony fishes, Fishing, Iran
http://isfj.ir/article-1-801-en.html
http://isfj.ir/article-1-801-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Effects of dietary carbohydrate levels on growth, survival and body composition in Caspian Kutum (Rutilus frisii kutum, Kamenskii, 1901)
85
94
FA
S.
Mohammadzadeh
N
H.
Noverian
N
H.
Ouraji
N
B.
Falahatkar
falahatkar@guilan.ac.ir
Y
10.22092/ISFJ.2017.110090
This study was carried out to evaluate the effects of dietary carbohydrate levels on growth performance and body composition of juveniles Caspian Kutum (Rutilus frisii kutum). Fish with initial average weight of 0.8±0.2g (±SD) were fed five isoproteic and isenergic formulated diets with different carbohydrate levels including 15, 20, 25, 30 and 35% in triplicate groups, 4 times daily according to their satiation for 10 weeks. Results showed that increasing the level of carbohydrate to 35% significantly increase some growth indices such as final weight (Wf), weight gain (WG), body weight increase (BWI), specific growth rate (SGR) and protein efficiency ratio (PER). In addition, result of body composition analysis showed that the increase in carbohydrate level lead to increase of protein level. So, the highest amount of protein was observed in fingerlings fed with diet containing 35% carbohydrate. In conclusion, the results indicate that increase in carbohydrate levels from 15 to 35% have not any negative effect on growth parameters and therefore, the level of 35% carbohydrate in the diet of 1-2g Caspian Kutum fingerlings can lead to better efficient in protein and desirable growth.
Feeding, Aquaculture, handmade food
http://isfj.ir/article-1-803-en.html
http://isfj.ir/article-1-803-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Histopathological Effects of Mercuric Chloride on Kidney and Intestine of Persian Sturgeon, Acipenser persicus fry
95
104
FA
S.
Mosafer Khoorjestan
Mosafer_Saeedeh@yahoo.com
Y
S.
Khodabandeh
N
Z.
Khoshnood
N
10.22092/ISFJ.2017.110091
To study the effects of mercuric chloride on kidney and intestine tissue of Persian Sturgeon, Acipenser persicus fry, fish exposed freshwater contain 15 ppb of mercuric chloride and control group were kept in non polluted freshwater for 48 hours. Histological studies of the processed tissues showed marked histological alterations than the control group that exposed only to freshwater. The pathologic lesions of the kidney included severely epithelial cell necrosis, lesion of epithelial cells from the basement membrane in the proximal and distal tubules. Proximal tubule also showed inflammation and incorporation of brush borders. Decrease of lymphoid and hematopoietic tissue observed. Pathologic lesions of intestine tissue were showed the epithelial necrosis, shrinkages of secretory follicule, lesion of epithelial microvilli in intestine. Results showed that low concentration of mercuric chloride can pose undesirable alternation in Persian Sturgeon fry, an economic and endangered species of the Caspian Sea.
Mercuric Chloride, Persian Sturgeon, Kidney, Intestine, Histopathology
http://isfj.ir/article-1-804-en.html
http://isfj.ir/article-1-804-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
The effects of dietary selenium on the oxidative stress of beluga, Huso huso, fed high copper
105
114
FA
M.
Mohseni
mahmoudmohseni@yahoo.com
Y
A.M.
Sotudeh
N
10.22092/ISFJ.2017.110092
The effects of dietary selenium (Se) were investigated on the oxidative stress of beluga, Huso huso, fed high dietary copper (Cu). The basal diet with high Cu (50mg Cu/kg) was supplemented with low (0mg Se/kg, HCuLSe), adequate (11mg Se/kg, HCuNSe) and 5× adequate (55mg Se/kg, HCuHSe) of Seandfed to fish with initial body mean (±SD) weight 14.96 ± 1.39g for 12 weeks. Basal diets containing adequate Se (11mg Se/kg) and adequate Cu (10mg Cu/kg) were used as controls (NCuNSe). Weight gain, specific growth rate and condition factor for fish fed the HCuHSe diet was significantly lower than fish that fed the other diets. Se and Cu content was positively correlated with dietary Se and Cu concentrations. Hepatic Se concentration was higher in fish fed the HCuHSe diet than fish that fed other diets. Thiobarbituric acid-reactive substances (TBARS) were significantly lower. Whereas, Glutathione peroxidase activity activities were significantly higher in liver tissue of fish fed HCuHSediet than those fish fed other diets. Plasma total immunoglobulin concentration and lysozyme activity were higherin fish fed the NCuNSe diet, followed by fish fed the HCuNSe diet, and were lower in fish fed the HCuHSe diet. The results indicated that optimum levels of Cu and Se ingestion induced an oxidative stress in beluga and improved their immune response. High dietary Se (5×adequate) supplementation reduced the oxidative stress and depressed the immune response of the fish. *Corresponding author
Beluga (Huso huso), Selenium, Copper, Growth rate, Oxidative stress
http://isfj.ir/article-1-805-en.html
http://isfj.ir/article-1-805-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Effect of reused water on growth index and survival rate of the Rainbow trout (Oncorhynchus mykiss)
115
124
FA
A.
Nekuie Fard
dr.anekuiefard@gmail.com
Y
H.
Hossainzadeh Sahhafi
N
A.A.
Motalebi
N
A.H.
Rastiannassab
N
Azadikhah D.
N
B.
Mostafazadeh
N
10.22092/ISFJ.2017.110093
This research was carried out to determine water reuse effects on growth indices of rainbow trout. For this reason 4 groups including: Control (with use of 100% fresh water), Test 1(rearing with use of 30% recycled water (with physical filtration and 10% fresh water), Test 2 (using of 10% recycled water (with physical filtration and 30% fresh water), Test 3(using of 100% recycled water) was done. Fish stock of each groups were done with 62 fish in each square meter and average (±SD) weight (15±1.5). The Growth index, SGR, CF, FCR, SR between different groups in each month was calculated and determined. In this research comparing the average of monthly growth increasment between different groups indicated that significant decrease of growth in test groups with control group as well as groups 1 and 2 with 3 groups. There were no significant differences in this factor between groups 1 and 2. FCR, SR and MR showed a significant difference between different groups. These results showed with increasing the replacement of water in rainbow trout culture ponds with recycled water cased significant decrease in SR and CF and significant increase in MR and finally decrease in production and density per unit area.
Oncorhynchus mykiss, reused water, growth index
http://isfj.ir/article-1-806-en.html
http://isfj.ir/article-1-806-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Diet composition, Trophic level estimation and Food consumption rate of Saurida tumbil (Teleostomi/Synodontidae) in the Bushehr coastal waters, Persian Gulf, Iran
125
150
FA
A.
Vahabnezhad
avn9400@gmail.com
Y
S.A.
Taghavi Motlagh
N
Javad
Shaebani
N
10.22092/ISFJ.2017.110095
In order To increase the knowledge of Saurida tumbil biology and ecology, an investigation of food habits of this species was conducted from October 2010 to September 2011 in Bushehr coastal waters, Persian Gulf. 485 fish stomach contents were studied. In this study the frequency of occurrence of prey, feeding intensity index, the index is empty stomachs, the mean Trophic levels and the rate of food consumption was calculated. Total three major taxonomic categories were identified in the stomach contents of this species and were weighted including arthropods (crustaceans higher crustaceans), mollusks (Cephalopod) and Teleostei and the results show that Teleostei was the most abundant prey item which including the family Carangidae, Nemipteridae, Synodontidae, Mullidae, Sphyraenidae, Leiognathidae , Haemulinae and Trichiuridae. The results showed that the highest intensity of feeding occurs in the fall and lowest in summer. S. tumbil also shows cannibalistic behavior in ecosystem. The mean trophic level for S. tumbil was estimated 4.64 for the first time in Persian Gulf, indicating that S.tumbil is largely carnivore and has a high rate of food consumption.
S. tumbil, cannibalistic, Consumption, Trophic level, Persian Gulf
http://isfj.ir/article-1-808-en.html
http://isfj.ir/article-1-808-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Study on Bentic organisms of Ghazal Ozan River in Zanjan Province
125
138
FA
Navan
Maghsoodi M.
Maghsoodi_m3@yahoo.com
Y
10.22092/ISFJ.2017.110094
Ghazal Ozen is one of the two main Caspian Sea catchments entering to the Sefidrood Dam. Sampling was done within 45 days from October 2009 till October 2011 on 3 stations. Benthic samples were fixed with %4 fomaline and were identified to the genus or species levels and investigated using to the Hilsenhoff biogogical index. In this study, 20 genera 7 orders were identified chironomidae as dominant family. Hilsenhoff Biological Index (HBI) for the 9 studied stations, were respectively, 5.4, 6.3, 5.46, 5.11, 5.13, 4.13, 4.25, 4, 4.23.The biological status of rivers based on HBI data was in the range of very good level. In the study, Chironomidae (30%), caenis sp. (16%), Baetis sp.(12%), Hydropsyche sp. (13%), Simmulium sp. (11%), Tabantus atratus and Sericostoma sp. (2%) and remaining benthos (14%) in Ghazan Ozan River. The results showed that the presence of organic matter pollutant has reduced oxygen levels and also affected the biota in Ghazal Ozan River.
Benthoses, Ghazal Ozan River, Hilsenhoff Index, Zanjan Province
http://isfj.ir/article-1-807-en.html
http://isfj.ir/article-1-807-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Parasitic infection in Hemiculter lucisculus, Liza auratus and Gasterosteus aculeatus of the Zardi River(Mazandaran)
151
156
FA
M.
Taghavi
mahdieh_taghavi@yahoo.com
Y
B.
Mokhayer
N
A.A.
Saeedi
N
H.
Mosavi
N
10.22092/ISFJ.2017.110096
A total of 76 specimens of three fish species including: Hemiculter lucisculus (41), Liza auratus (16) and Gasterosteus aculeutus (19) were caught using handnet from 3 station in Zardi River in the south-eastern costal of Caspian Sea at autumn and winter 2009 and spring 2010. The fishes were transported alive to the laboratory of Shahid Rajaee Site. They were killed by cutting their spinal cord and their length and weight were measured. The eye, skin, gill, digestive system, muscle and other organism's of the specimens were examined for parasite infestation. Thirteen parasites species were identified. Ichthyobodo necator was collected from skin of Hemiculter lucisculus and Liza auratus. Ichthyophthirius multifiliis was collected from skin or branchia of Liza auratus and Gasterosteus aculeutus, Chilodonella sp. was collected from skin of Gasterosteus aculeutus, Trichodina sp. from skin and branchia of Hemiculter lucisculus, Liza auratus and Gasterosteus aculeutus, Tetrahymena sp. was collected from skin of Gasterosteus aculeutus , Myxobolus sp. from branchia of Hemiculter lucisculus, Dactylogyrus sp. from branchia of Hemiculter lucisculus and Gasterosteus aculeutus, Gyrodactylus sp. from skin or branchia of Gasterosteus aculeutus, Discocotyle sp. and Diplozoon sp. from branchia of Hemiculter lucisculus, Diplostomum spathaceum from eyes of Hemiculter lucisculus, Saccocoelium obseum from intestine of Liza auratus and Corynosoma stromosum from abdominal cavity of Gasterosteus aculeutus. In present study Hemiculter lucisculus was as a new host for Ichthyobodo necator, Myxobolus sp., Discocotyle sp. and Diplozoon sp. also except Saccocoelium obseum from Liza auratus and Corynosoma stromosum from Gasterosteus aculeatus, the other parasites were reported for the first time in Iran. *Corresponding
Parasites, Zardi River, Caspian Sea, Fish
http://isfj.ir/article-1-809-en.html
http://isfj.ir/article-1-809-en.pdf
IFSRI
ISFJ
1026-1354
2322-5998
21
4
2013
1
1
Short term preservation of spermatozoa of rainbow trout (Oncorhynchus mykiss)
157
164
FA
M.
Pourkazemi
Y
A.
Shakibi Daryakenari
N
M.R.
Kalbasi
N
H.
Abdolhay
N
S.
Baradaran Noveiri
N
10.22092/ISFJ.2017.110097
Short term preservation of spermatozoa of salmonid fishes for practical use, increasing of reproduction potentials and reducing of expenses, has an important role in hatcheries. In this study spermatozoan motility and fertilization rate of 29 commercially cultured rainbow trout were examined. Semen was collected by abdominal stripping at the volume of 4.49±1 (mean±SD) ml and sperm concentration was determined by heamocytometer following 1:600 dilution with saline solution (0.9% NaCl). To find the effects of preservation time on fertilization capacity, samples were diluted with extender (Glucose 54gL-1, Tris 0.5gL-1, DMSO 9% , Egg yolk 20%) and held at 3 different cool conditions : room temperature (+15°C), refrigerator (+5°C) and crashed ice (+ 1-2°C) and sperm motility followed up each 30 mins until complete ceasation. Fertilization, hatching and larval stage rate were determined after 24, 48 and 72 hours. Results showed that the motility of sperm samples held at 15 °C and 5°C were completely ceased after 2 and 24 hours respectively. However it lasted up to 144 hours at1-2°C.Fertilization, hatching and larval stage rates of sperm samples hold at 1- 2°C were 68.45% , 62.1% and 58.2% (after 24 hours), 53.2% , 43.2% and 40.9% (after 48 hours) and 36.6% , 31.6% and 23.7% after 72 hours, respectively, and all showed a significant decrease compared with control (90.32%, 83.27% and 81.12% respectively). Comparing the results showed that holding samples in test tubes at 1-2°C is the best method for short term storage of rainbow trout spermatozoa.
Spermatozoa, Short term preservation, Oncorhynchus mykiss, Motility rate, Fertilization rate
http://isfj.ir/article-1-810-en.html
http://isfj.ir/article-1-810-en.pdf