We assessed concentration of heavy metals (Cu, Fe, Hg and Zn) in the muscular tissue of benthic species Himantura gerrard and pelagic species Selar crumenophthalmus caught from the Persian Gulf during winter 2008. After biometrical measurements, the muscle tissues of  51 randomly selected samples were separated from 86 stations. Specimens were digested using the wet method and the concentrations of Cu, Fe and Zn were determined using Atomic Absorption Spectrophotometer (flame method) and also concentration of mercury was determined using Mercury Analyzer device. The mean concentration of heavy metals in muscle tissues of Himantura gerrardi were as follows: Fe: 74.78; Hg: 0.77; Zn: 875.99 and Cu: 3.5. However, these values for muscle tissues of Selar crumenophthalmus were: Fe: 25.07; Hg: 0.20; Cu: Zn: 15.54 and Zn: 2.31f.lglg dry weight. Results showed that concentration of the selected heavy metals in H gerrardi, were significantly higher than S. crumenophthalmus (P O.OS).