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CRISPR-Cas technology in aquaculture: A review
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Mahmoud Hafezieh   1, Pezhman Hosseini Shekarabi1 , Mohammad Akhavan Bahabadi1 |
| 1- AREEO |
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Abstract: (511 Views) |
| The progress of the aquaculture industry is facing many challenges, including the spread of infectious diseases and antibiotic-resistant pathogens, reduced survival, reduced fertility, slow growth, escape of farmed fish to natural ecosystems, and environmental pollution. Today, the use of CRISPR-Cas technique is considered as a potential solution to solve these challenges through genome editing. In the CRISPR system, the nuclease 9 or Cas9 enzyme is a powerful and efficient tool for molecular editing of DNA to reveal desired traits in the host. In this context, several studies have been conducted on different aquatic species to investigate desirable traits related to aquaculture, including suppressing the myostatin gene (increasing somatic growth of the body), biosynthesis of fatty acids, stimulation of body pigmentation, and production of fish with less intermuscular bones. In terms of reproduction traits, this technology has been used for the genetic engineering of sex cells and sex reversal. In the field of aquatic health, this genome-based breeding system has successfully produced fish resistant to infectious diseases, especially viral diseases, on a laboratory scale. Also, editing genes related to antimicrobial peptides by CRISPR-Cas9 can improve the innate immune system and increases the resistance of fish against infectious diseases. In general, using the CRISPR-Cas system is an effective approach to manipulate target genes and improve economic traits in different aquatic species in line with genetic modification programs. This review study provides a comprehensive view of CRISPR-Cas technology and its potential in genome editing to target genes associated with economically valuable traits in fish to overcome some limitations and challenges of promoting sustainable aquaculture. |
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| Keywords: CRISPR, Cas9, Sustainable aquaculture, Genome editing, Molecular genetics |
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Full-Text [PDF 1252 kb]
(213 Downloads)
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Type of Study: Applicable |
Subject:
ژنتيك و اصلاح نژاد
Received: 2024/06/20 | Accepted: 2024/08/31 | Published: 2024/10/2
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