|
|
|
|
|
|
|
|
|
|
|
RSS |
|
|
|
 |
|
|
|
|
 |
Search published articles |
 |
|
Showing 2 results for Ghavam Mostafavi
N. Pourang, F. Parafakndeh, H. Moazami, D. Agha Aligol , P. Ghavam Mostafavi , M. Mahmoudian,
Volume 27, Issue 1 (5-2018)
Abstract
In this research, the potential use of five hard parts (otolith, scale, eye lens, dorsal spine, vertebral bone) of Rutilus kutum for populations discrimination and biomonitoring as well as their usability as biological archive in the southern Caspian Sea was investigated. Two soft tissues (muscle and liver) were also analyzed for assessment of the possible risk of consumption and use as biomonitoring tool, respectively. A total of 60 specimens were collected using beach seine from three sampling sites (adjacent to the estuaries of the rivers Sefidrud, Tajan and Grorganrud, respectively) in the Southern Caspian Sea in May 2016. In total, the levels of 13 major and trace elements in the samples (the seven soft and hard tissues) was measured using PIXE (Proton Induced X-ray Emission) and µ-PIXE methods. Minor similarities among the tissues were observed in the case of eye lens (for P, S and Zn) and liver (for Cl, Fe and K). There were no significant relationships between the elements and biological characteristics (weight, total length and standard length). Based on the results, it seems that all the studied hard parts, except for eye lens, may offer alternatives for biomonitoring of Ca, Cl, Fe, K and S in the southern Caspian Sea. Considering Sr level in otolith, no significant differences could be found between the sampling sites 2 and 3. So it can be concluded that the specimens collected from the two sites are probably from the same population. Similar results were also obtained based on the results concerning K accumulation in scale. It can be concluded that scales may provide an alternative structure to otolith for stock discrimination of R. kutum in the southern Caspian Sea. Among the 10 elements that were detectable in the muscle using the PIXE method, only in the case of four of them (i.e. Zn, Cu, Fe and Mn) maximum allowable concentrations for human consumption have been defined, which in all the cases the mean concentrations in the present study were far below the recommended limits.
Sh. Aghamirkarimi, A. Mashinchian Moradi, I. Sharifpour, Sh. Jamili, P. Ghavam Mostafavi,
Volume 27, Issue 5 (1-2019)
Abstract
The current study has determined the toxicity effects of copper nanoparticles (CuNPs) on the some vital organs such as gill, liver and kidney of Caspian Roach; Rutillus rutillus caspicus. For this purpose, 120 fishes were used as experimental fishes and exposed to 0.1, 0.2 and 0.5 mg/L of Cu nanoparticles for 21 days, and 30 fishes as control. The mean water temperature of the aquaria was 22±2 ºC, dissolved oxygen 5.2 mg/L, pH at 7±0.004 and the concentration of Calcium Carbonate was 270 ppm. On the 7, 14 and 21 days after exposing the fishes to CuNPs, three fish were randomly selected from each aquaria, sacrificed and samples from their liver and blood were taken. Changing in antioxidant enzymes level were determined by evaluation of superoxide dismutase (SOD) and catalase (CAT) activities in the blood of fish. In first week, the samples that exposed to 0/1 mg/L of CuNPs concentration had more activities in SOD and CAT levels (p<0.01) but other treatments (0.2 & 0.5 mg/L) didn’t have any increase in enzyme activities. The liver microscopic sections were prepared and stained by H&E method and examined by light microscope which showed histological alternations in the liver tissues. Histological changes in liver included blood congestion in the central veins, cytoplasmic vacuolation of the hepatocytes, cellular hypertrophy, congestion in the blood sinusoids and necrosis of the hepatocytes and nuclear pyknosis. The degree of damages was more intensive at higher CuNPs concentrations. Evaluation of these changes could be useful in estimating the harmful effects of CuNPs. The result of the study showed that CuNPs could cause decrease in antioxidant enzyme activities and severe damages in the liver tissues of Caspian roach; Rutillus rutillus caspicus and have lethal effects for fish.
|
|
