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Showing 3 results for Hassanzadeh Saber
Mohammad Hassanzadeh Saber,
Volume 32, Issue 4 (11-2023)
Abstract
By broodstocking and propagating of specific pathogen-free (SPF) rainbow trout in Iran, the need to create a cryopreserved sperm bank from the breeders was deemed necessary. In this study, after evaluation of four different extenders for rainbow trout sperm cryopreservation, the best extender (0.6M sucrose and 8% DMSO) has been selected. The sperm samples were obtained from 24 breeders, including four breeders from each of the selected specific pathogen-free farms of the country in Mazandaran and West Azerbaijan provinces (Sarshar, Maroufi, Fakhari, Hadidi, Malekitabar, and Qorbani) were cryopreserved after qualitative evaluation. The fertilization test of sperm samples on the day of sampling and comparison of their fertilization percentage with frozen-thawed sperms in three time intervals (4, 24 and 48 hours) did not show any significant differences between the sperm of the control group and the frozen-thawed samples. The results indicated that the method used in this research can be well used in cryopreservation the sperm of SPF rainbow trout breeders and if needed, the sperm obtained from the best breeders can be transferred to other centers with this method.
E. Abdollahzadeh, O. Jafari, M. Hassanzadeh Saber,
Volume 33, Issue 2 (6-2024)
Abstract
Listeriosis, an important illness with a high mortality rate (up to 30%), is caused by Listeria monocytogenes. Nowadays, there are several reports that display the prevalence of L. monocytogenes in fisheries products. Therefore, it is important to investigate and control the expression of virulence genes and adhesion-related genes in different strains of this bacterium in foodstuffs. In this study, the expression levels of inlA, hly, lmo1634, lmo1847, sigB, and prfA genes in two isolates (food and clinical) were investigated under the influence of NaCl and lime juice in the shrimp broth. The results of the independent t-test for the prfA gene demonstrated that the expression level in the treatments of clinical strain was significantly higher than the food strain (p<0.05). Similarly, the average expression of both adhesion genes (lmo1847 and lmo1634) and stress gene (sigB) in the studied treatments for the clinical strain was significantly higher than the food strain. In both strains, the role of lime juice in increasing the expression of virulence and adhesion genes was significantly higher than in NaCl treatments (p<0.05). Overall, it can be stated that the transcription level of virulence- and adhesion-related genes upon exposure to preservatives may be different in the various L. monocytogenes isolates.
Omid Jafari, Maryam Nasrolahpourmoghadam, , Mohammad Hassanzadeh Saber,
Volume 33, Issue 3 (8-2024)
Abstract
Fish population segregation through diagnostic morphometric traits has a great importance in fish conservation and fisheries management programs. In the current study, morphometric variations of 31 morphometric characteristics of Alosa braschnikowi was investigated in the Southern Caspian Sea in three provinces including Guilan, Mazandaran and Golestan, during winter 2021. The average standard length (±SD) was 231.73±4.38, 231.52±4.14 and 200.93±32 in Guilan, Mazandaran and Golestan, respectively. The results obtained from the one-way ANOVA showed a significant difference (p<0.05) in 17 out of 31 morphometric traits. The PCA showed that the investigated areas can be distinguished through nine components, so that the main changes in the first component (PC1) are in the head area while variations in the pectoral and dorsal fins were identified as important through the second component (PC2). Investigating the growth pattern of A. braschnikowi illustrated a negative allometric growth for Anzali (b=2.78) and Sari (b=2.61) regions while Miankaleh (b=3.12) had a positive allometric growth pattern. Furthermore, condition factors estimation revealed a better nutritional condition in Anzali (CF=1.10) and Miankaleh (CF=1.09) compared to Sari region (CF=0.71). Morphometric-based clustering showed that there are at least two separate populations of A. braschnikowi in the studied areas of the Southern Caspian Sea. Due to the evenness in sampling season and way of sampling, it seems that the environmental features in each region have caused the ensuing high morphological diversity in A. braschnikowi, however, investigations through molecular methods are needed for this claim. Considering the high rate of morphometric variations in Alosa genus in the Caspian Sea, the usage of machine-learning based analytical methods is highly recommended for future studies.
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