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Showing 29 results for Jafar
F. Mashayekhi , Y. Morady , A Ashraf Gohari , M. Jafar, G. Zarea, Alireza R.g., Volume 22, Issue 1 (4-2013)
Abstract
The effect of three different packaging methods including Modified Atmosphere Packaging (MAP), Vacuum Packaging and normal Packaging was investigated on the quality of Nile tilapia fresh fillets stored in the refrigerator's temperature. The packaged samples were examined for 10 days with regard to the changes in chemical (TVN, PV, pH), microbial (total viable count) and sensory evaluations. The results indicated that the samples packed in MAP condition had higher quality than that of other methods at the end of the storage period. In addition, the slower destructive impacts and microbial growth was observed in MAP. The results of present study suggest that packaging tilapia under MAP conditions results in the increase in the durability, storing, and distribution period for fillets.
A. Jafarpour , A. Alinejad , S. Yeganeh , R. Safari, Volume 22, Issue 2 (7-2013)
Abstract
Fermented sausage is a favorite kind of meat-product that has allocated great proportions of meat consumption in the world. For the first time in Iran in this study the production of Fermented sausage from minced meat of common carp was assessed by means of lactic acid bacteria at different incubating temperatures as 15, 25, and 35°C. To prepare the fish sausage, common carp mince was grounded and mixed with NaCl (3%), glucose (3%) and lactic acid bacteria at 5 log CFU/g and afterward were incubated for 48 h. During the incubation of fish sausage, microbiological tests, moisture and protein content, and TVB-N were measured. According to the results, the higher temperature of 35°C stimulated the rapid growth of lactic acid bacteria, resulting in a rapid decline in pH, and consequently suppressed the growth of pseudomonas, Micrococcaceae and Enterobacteriacea.
A. Hasanpour Fattahi , H. Jafaryan, A. Khosravi, D. Abdollahi Arpanahi , Volume 23, Issue 2 (7-2014)
Abstract
This study was conducted to evaluate the effects of dietary autochthonous Saccharomyces cerevisiae and Aspergillus niger on the growth performance, survival rate, ammonia excretion, immune response and the intestinal microbiota of juvenile beluga sturgeon (Huso huso). Beluga juveniles with average (±SD) weight of 31.8±2.81 g were randomly allocated into 12 oval tanks (1000 l) at a density of 30 fish per tank and triplicate groups and were fed either with a basal control diet (no supplemented with probiotic) or with the basal diet supplemented with S. cerevisiae and A. niger (2×106, 4×106 and 6×106 cells g-1). After 8 weeks of feeding on the experimental diets, growth factors, survival rate, ammonia excretion, immunity parameters and gut microbiota were measured. The results indicated that dietary supplementation of 6×106 (cells g-1) S. cerevisiae and A. niger significantly improved growth indicators, survival rate, immune parameters and ammonia excretion compared to the control treatment. Additionally, total autochthonous intestinal fungus probiotic and Lactobacillus spp. counts were affected by dietary treatment. The results showed that dietary supplementation of S. cerevisiae and A. niger (6×106 cells g-1) had positive effects on growth and immunity factors in cultured juveniles beluga.
D. Abdollahi Arpanahi , H. Jafaryan, M. Soltani , H. Gholipour Kanani, Volume 23, Issue 4 (1-2015)
Abstract
The effect of dietary containing of Bacillus subtilis and Bacillus licheniformis in three groups including commercial, commercial-indigenous and indigenous was investigated on the immune parameters (glucose, albumin, total protein, lysozyme, cortisol, immunoglobulin M (IgM)) and the intestinal flora of white leg shrimp (Litopenaeus vannamei) post larvae. The shrimp were fed for 60 days with four different diets: control (without probiotics), diet T1 supplemented with 1.5×106 CFU g-1 commercial probiotic, diet T2 with 1.5×106 CFU g-1 commercial-indigenous probiotic, diet T3 with 1.5×106 CFU g-1 indigenous probiotic. At the end of experimental period, the levels of biochemical parameters (glucose, total protein, lysozyme, cortisol, IgM) of shrimp fed probiotic diets were significantly higher than in those shrimps fed the control diet for 60 days. However, albumin concentrations showed no significant difference between the experimental treatments and the control, but increased by 1.19, 1.15 and 1.14 after 60 days of feeding with diets T1, T2 and T3, respectively. Likewise, population density of Bacillus bacteria counted in digestive tract of shrimps treated with probiotic were significantly higher than the control group. Results of this study indicated that the addition of probiotic bacili can improve immune parameters and modulates intestinal microbiota of shrimp (L. vannamei) post larvae.
S. A. Jafarpour, R. Esfahani , S. M. Jafarpour, Volume 25, Issue 2 (7-2016)
Abstract
The aim of this study was to optimize the nanoencapsulation of fish Omega 3 fatty acids with use of complex coasevation technique which was performed by using response surface methodology (RSM) in the central composite design (CCD). Nanoparticles containing omega-3 fatty acid were produced by Rainbow trout skin gelatin and Arabic gum as walls using complex coacervation technique. The effects of three independent variables as fish gelatin concentration (33-66%), the concentration of omega-3 fatty acids (10-30%) and the homogenizer speed at three levels (10000, 15000 and 20000 rpm) on the dependent variables such as particle size, oil level, encapsulated oil and nanoencapsulation efficiency were evaluated. The results showed that the homogenizer speed significantly had the greatest impact on the particle size (P<0.01). Determination of the nanoparticle surface oil indicated that surface oil was higher in the treatments with higher levels of omega-3 fatty acid than the treatments with lower concentration of omega-3 fatty acid. Nanoencapsulation efficiency of produced powder was recorded at about 41.98-74.69 %. Therefore, it is recommended to use 33% fish gelatin with 30% omega-3 fatty acids at 20000 rpm for production of omega-3 fatty acids nanocapsules with desired properties.
S. Nazari, V. Jafari, M. Pourkazemi, H. Kolangi, H.a. Abdolhai, Volume 25, Issue 3 (10-2016)
Abstract
In this study the rainbow trout (Oncorhynchus mykiss) myostatin gene (MSTN) for detecting gene derived SNPs and performing genetic association study between MSTN polymorphisms and growth traits were analyzed. Twenty broodstocks of rainbow trout in Genetic and Breeding for coldwater Fishes center in Yasouj for generating full sibling were selected and tagged and then juveniles were raised at the ponds until 5 months post-hatching. DNA extract based on standard method and these individuals selected randomly for molecular analysis. Two single nucleotide polymorphisms (SNPs: g.660T>C and g.1904C>A) in intron 1 and 2 regions of the MSTN gene in rainbow trout by were identified DNA sequencing and polymerase chain reaction single-stranded conformational polymorphism (PCR-SSCP) methods. The assessment results demonstrated that 6 haplotypes were revealed in this center. Moreover, association analysis indicated that the genotypes of SNP g.1904C>A are associated with the length and body weight at 90 and 150 days of age (P< 0.05), whereas no significant association was found between the SNP g.660T>C and growth traits. Our results provided evidence that SNPs in MSTN gene may be associated with length and body weight at fingerling age and MSTN gene may be used as potential candidate gene for marker-assisted selection (MAS) in rainbow trout breeding program
H. Taghavi Jelodar, M. Asgari, H. Fazli, N. Jafari, Volume 26, Issue 1 (4-2017)
Abstract
To determine the fecundity and population composition of Gammarus (Pontogammarus maeoticus (Gurianova,1951) ) in coast line of the Caspian Sea, monthly sampling was conducted in ten stationg (between Babolsar to Fereydunkenar) from August 2013 to April 2014.A total of 1906 P.maeoticus speciments, male populationcomposition (673), female (873) and immature (360), respectively. Kruskal-Wallis test based on the average number of Gammarus in stations (χ2 = 14.8; p<0.011) and months (χ2 = 17.6; p<0.040) showed significant different. The average length of immature, male and female was 4.1, 8.5 and 8.3 mm and weight 0.0068, 0.0303 and 0.0282, respectively. Males have more weight and length than the female and the mean length and weight of the two sexes was statistically significant (length: t-test=2.05; p<0.041 Weight: t-test=2.81; p<0.005, respectively). Gammarus Length and weight equation was very strong based on exponential relationship (p<0.001; R2= 0.80) and slope of line b was 2.1205. The minimum average fecundity was 59/0±0/6 and a maximum of 71/0±4/16. Kruskal-Wallis test between the mean fecundity in different months showed a significant difference (p<0.001).The results show that the overall ratio of male: female was 77/0: 1 which showed completely dominant of females (p<0.001).Also compared the frequency of male and female at different months showed that only in August and March females are dominant statistically (p<0.05).
N. Koosej, H. Jafariyan, A. Rahmani, A. Patimar, H. Gholipoor, Volume 26, Issue 1 (4-2017)
Abstract
The objective of this study is to evaluate and compare the concentration of metals, lead, nickel, iron and copper in muscle tissue of White shrimp (Metapenaeus affinis) in three regions (Qeshm, Khamir port and Laft port) in Hormozgan Province. Sampling was conducted in summer and winter 1394. After the 180- captured biometric samples, muscle tissue was isolated. The preparation and analysis of samples was performed according to standard methods MOOPAM. The results indicated that the studied concentration in muscle tissue of White shrimp at sample locations showed significant difference statistically. In terms of concentration in the muscle tissue of White shrimp in the study area between summer and winter significant difference was observed, statistically (p<0/05). As both in summer and winter concentrations of lead, nickel, iron and copper in muscle tissue of White shrimp in Qeshm is greater than the other two regions. The studied concentration in muscle tissue of White shrimp in Qeshm, Khamir port and Laft port international standards is less than the limit which indicates that the health of aquatic ecosystems contamination is not a problem.
M. Asgharnia, S. Yeganeh, S. A. Jafarpour, R. Safari, Volume 26, Issue 3 (9-2017)
Abstract
The aim of this study is to chemically hydrolyze of silver carp (Hypophthalmichthys moltirix) viscera and the effect of obtained peptones as a nitrogen source for culture of Listeria monocytogenes. Silver carp viscera were provided by a local market in Sari, Northern Iran. After freeze-thawing of Viscera at 4 °C was used for hydrolysis. Acidic and alkaline hydrolysis was done at two pH 3.3 and 12, and two temperatures of 70 and 85 °C. At the end of hydrolysis DH% (hydrolysis degree) and protein content were measured. Then obtained peptones from these 4 treatments (three replicates for each treatment) used as Listeria monocytogenes culture media at 48 hours and compared with BHI culture media. Results showed that maximum and minimum protein concentration were related to alkaline hydrolysis at 85 °C and acidic hydrolysis at 70 °C, respectively (P<0.05). The highest DH% was related to alkaline hydrolysis at 85 °C and the lowest of it was related to alkaline hydrolysis at 70 °C (p<0.05). Growth of Listeria monocytogenes in acidic and alkaline produced treatments at 70 °C had no significant differences compared to control (BHI) except at 36 and 48h. But at 85 °C had differences at all hours significantly (P<0.05). This study showed that alkaline hydrolysis in higher temperature is better than acidic hydrolysis and growth of bacteria in fish peptone could be done as well as commercial cultured media. Due to usage of fish viscera and alkaline method with low processing cost, it‘s economically suitable.
Ali Jafarpour, Farzaneh Yazdanprast, Reza Safari, Volume 26, Issue 3 (9-2017)
Abstract
In this study, the effects of inoculation of two lactic acid bacteria including Pediococcus pentosaceu and Lactobacillus plantarum were evaluated on the fermented fish sausages produced from Common carp, as a model, at incubation temperature of 35˚C. Common carp fermented sausages were prepared from minced fish, salt (3%), glucose (3%) and the combination of two starter cultures of bacteria (6 log CFU/g) and fermentation occurred during the 48 h incubation. Soluble peptides, pH, phsycrophilic and lactic acid bacterial counts, proximate composition (protein, lipid, moisture and ash), texture profile analysis and color of fermented sausages were measured and compared to the non-inoculated sausages (the control group) at 0, 24 and 48 h after incubation. According to the results, lactic acid bacteria showed rapid growth after 48 h fermentation which led to the significant decrease in pH from initial values of around 6.9 to 4.4 (P<0.05) and the significant decrease in growth and proliferation of phsycrophilic Pseudomonas (P<0.05). The amounts of soluble peptides significantly increased in sausages that were inoculated with the mixed culture as compared to the control group (P<0.05). In terms of texture, the hardness and chewiness of inoculated sausages were higher than that of the control group (P<0.05). In terms of color parameters, the lightness (L*) and redness (a*) of both types of sausages were significantly increased after incubation, whereas the yellowness (b*) was significantly decreased (P<0.05). It can be concluded that fermented sausages inoculated with mixed starter cultures had more favorable physiochemical characteristics and textural properties as compared to the control group.
A. Jafarpour, E. Sharifi, M.h. Hosseini , Volume 26, Issue 6 (3-2018)
Abstract
The aims of this study were to evaluate the effects of three independent variables including the percentage of total biopolymer (6, 8 and 10%), fish oil content (2 and 3%) and concentration of clove oil (0, 2000 and 3000 ppm) on two dependent oxidative variables including the peroxide value (PV) and thiobarbituric acid (TBA) during 3 months of storage at 4 and 25°C. Evaluating the results after three months of storage indicated that the amounts of PV and TBA of the encapsulated fish oil were significantly lower than those of the control group (P<0.05). Furthermore, the amounts of PV and TBA of the encapsulated fish oil stored at 4°C were lower than the amounts of PV and TBA of the encapsulated fish oil stored at 25°C. The amounts of TBA of the encapsulated fish oil containing clove essential oil were decreased by increasing the amounts of clove essential oil (P<0.05) and the stability of oil was decreased by increasing the percentages of fish oil (P<0.05). Overall, it can be concluded that using 10% biopolymer, 2% fish oil and 2000 ppm clove essential oil were capable to preserve the fish oil stability during three months of storage at 4°C, whereas using 8% biopolymer, 3% fish oil and 3000 ppm clove essential oil were effective in preserving the fish oil stability during three months of storage at 25°C.
F. Mehrabi, A. Jafarpour, Gh. Nematzadeh, Volume 27, Issue 1 (5-2018)
Abstract
Carbon and nitrogen sources are considering as the essential substances for microalgae growth. In this study the effects of sodium Nitrate (NaNO3) and sodium bicarbonate (NaHCO3) on the growth rate of N. oleoabundans microalgae in BBM culture medium were investigated. In this regards, different concentrations of NaNO3 as 0, 0.5. 1.0, 2.5, 5 and 10 and NaHCO3 as 0, 0.25, 0.5, 0.75, 1.0 and 1.25 in BBM during 21 days were tested. According to the results, by application of 10 mM NaNO3 the highest number of microalgae as (84.66±0.577)× 106 cells/mL was recorded and in case of nitrogen absence no increase in cell numbers was observed (p <0.05). However, by increasing of the concentration of NaNO3, the lipid production was decreased which the highest amount of lipid was recorded as 1.32±0.27 gL-1 without nitrogen addition (p <0.05). In case of NaHCO3, by addition of 1.25 gL-1 sodium bicarbonate the highest cell number as (96±1.00)× 106 cell/mL was recorded whereas in its absence the lowest number of (2.00±0.00)× 106 cell/mL was obtained (p <0.05). Similarly, by addition of higher amounts of NaHCo3, the lipid production was decreased as its highest amount as 0.3±0.144 gL-1 was produced at level of 0.25 gL-1 of NaHCO3 (p <0.05). Considering the positive effects of NaHCO3 on growth of N. oleoabundans on BBM, it is recommended to use it for mass production of this microalgae.
N. Mashaii, F. Rajabipour, M. Jafari, M. Mohamadi, H. Sarsangi, Volume 27, Issue 2 (7-2018)
Abstract
The different aspects of aquaculture and biology of Tilapia have surveyed since 2008 at National Research Center of Saline Water Aquatics. In the present study, some limnological factors of earthponds and indoor tanks of Tilapia culture were investigated during the breeding season of 2016. In the earthponds, the minimum and maximum of water temperature ranges were 16.6-27.5 °C and 20.5-31 °C, air temperature 2-27 °C and 30-48 °C, dissolved oxygen in the morning and afternoon 2.11-10.19 mg/L and 7.06-14.64 mg/L, pH in the morning and afternoon 8.97-10.9 and 9.04-13, the depth of transparency 40-105 cm, salinity 7.94-8.82 ppt and ammonium density less than 0.05 mg/L to 0.4mg/L. The most abundant phytoplankton and zooplankton were Chlorophytes, Diatoms and Rotifers, and dominant macrobenthos were Chironomids. There was significant positive correlation between pH value with ammonium density and body length and weight, water temperature with total length, and negative correlation between dissolved oxygen and temperature and pH (p<0.05). In the indoor tanks, the ranges of water temperature was 24-29°C, the minimum and maximum air temperature 16-30°C and 30-43 °C, dissolved oxygen 1.65-8.38 mg/L, pH 7.25-9.37, salinity 7.7-7.94 ppt, ammonium 0.16-5.0 mg/L. There was significant positive correlation between water salinity with body weight and length and negative correlation between the amounts of dissolved oxygen with temperature, pH and total length (p<0.05). Growth proceeding of red Tilapia was more favorable in the earth pond. Tilapia culture in earth ponds has advantages especially for using water biomass by fish and lower costs of the structures, however indoor systems are preferable because of the possibility of two growing periods per year, water conservation and environmental considerations.
F.z. Jafari, A.a. Hedayati, S.a, Hosseinifar, A. Jafar Nodeh, T. Bagheri, Volume 27, Issue 2 (7-2018)
Abstract
The aim of this study was to investigate the protective effect of pre-treatment of bacteria Pediococcus acidilactici and oligosaccharide raffinose on hematological indices of Goldfish (Carassius auratus) during exposure to nanosilver. Accordingly, 300 fish were fed for 6 weeks in four treatments. After the end of the feeding period, 50% of the lethal nano-silver concentration (0.5 mg/L) was added to the treatments for the 14-day exposure, and blood sampling was performed. Neutrophil, monocyte and RBC indices did not differ significantly in treatments (P>0.05), but the number of white blood cells, hemoglobin, hematocrit, eosinophil were significantly different with the control group in different treatments (P<0.05). The results of eosinophil showed that exposure to silver nanoparticles caused a significant effect of dietary supplements and increased the amount of blood eosinophil (P<0.05), so that the bacteria and synbiotics have the same function, but raffinose had a growth-enhancing effect. The study of hematocrit index showed that dietary supplements undergoing exposure to silver nanoparticles had a significant effect on the hematocrit index and its increase (P<0.05), so that the bacteria and raffinose had the same function, but synbiotic was able to produce the increasing effect. The results of hemoglobin showed that exposure with silver nanoparticles had a significant effect on hemoglobin (P<0.05). Food supplements had a positive effect on goldfish immune system and increased hemoglobin in exposure to nanosilver. The raffinose had the same performance, but the bacteria had an increasing effect. According to the results, the dietary supplement of the bacteria had the highest effect on the hemoglobin indices and it has been effective in co-administration with other supplements in the blood monocyte index. Raffinose supplementation had the greatest effect on RBC, and eosinophilic indices. Among the supplements, the synbiotic food with effect on hematocrit, white blood cell, neutrophil, biochemical among food supplements has more effect.
Z. Eskandari, A. Jafarpour, A. Motamedzadegan, Volume 27, Issue 4 (10-2018)
Abstract
Fish gelatin, by benefiting the lower melting point, quicker mouth melting and presenting elastic characteristics is considered as the most proper substitute for mammalians gelatin and as a Halal food item. The aim of this study was to evaluate the effects of temperature at three levels of 30, 40 and 50 ºC, enzyme at 0.055%, 0.01% and 0.1% (w/w) levels and pH at 2, 3 and 4 levels on the quality of extracted gelatin from Huso huso fish skin. Accordingly, the gel strength in form of breaking force and distance, yield and color (L*a*b*) and whiteness of resultant gelatin were determined. According to the results, the highest gelatin yields as 21.65% was related to the enzymatic assisted method at 50 ºC, 0.01% enzyme at pH=2, whereas it was obtained as 10% at the chemical extraction method. Based on the results, despite from temperature, by increasing of pH and enzyme percentage, the firmness of gelatin was increased (p<0.05). The highest firmness was recorded at 30 ºC, 0.1% enzyme at pH=4. The color data revealed that lightness (L*), redness (a*) and whiteness of enzymatic extracted gelatin powder was higher that the chemical extracted one (p<0.05). The highest lightness was observed at 40 ºC, 0.055% enzyme at pH=3. In conclusion, by application of 30 ºC and 0.1% enzyme at pH=4 the highest gelatin with the highest firmness is achievable, while using 50 ºC and 0.01% enzyme at pH=2 could results the gelatin yield.
O. Jafari, A.a. Hedayati, M. Zeinolabedini, H. Poorbagher, R. Ghorbani, H. Abdolhay, Volume 28, Issue 1 (5-2019)
Abstract
The present study was performed with the aim of investigation in phenotypic variations of Cyprinus carpio as an economically worth species in south basin of the Caspian Sea. In winter of 2015, totally 74 specimens were collected from Anzali, Miankaleh and Gomishan and the morphometric information were extracted. The obtained results of ANOVA were indicated a high rate of phenotypic variations (23 out of 25). PCA detected HH1 (head height character) as the most important feature in making variations among populations. Cluster Analysis classified all samples in three major clades. So it can be concluded that there is at least three separate stocks of common carp in the southern basin of the Caspian Sea which it is high recommended that should be considered in stocks management in order to prevent subsequent problems including inbreeding and reduction in genetic fitness.
N. Badfar, A. Jafarpour, Volume 28, Issue 1 (5-2019)
Abstract
One of the problems associated with surimi, which is made from fresh water fish, is its low whiteness value compared to the marine fish species, mainly due to the redness of its muscles. In this study, hydrogen peroxide at concentrations of 1, 2 and 3% along with other variables such as water to mince ratios of 1:2, 1:3 during 1, 2 and 3 washing cycles was applied in order to improve the color quality of surimi obtained from Silver carp mince. According to the color test and the amount of utilized water for washing, in the one and two washing cycles, except for treatment 4 (1% H2O2 concentration, mince: water ratio of 1: 2, 1 washing) and treatments 10 (1% H2O2, mince: water ratio of 1: 2, twice washing) which resulted lower whiteness, the other treatments can be considered as an effective treatment to improve the color of surimi. According to the results, treatments 4 and treatment 10 with a numerical value of whiteness index 68.20 and 68.37, respectively, were significantly different compared to other treatments within their groups (P<0.05). In terms of 3 washing cycles process, no significant difference was observed among the numerical values of the white index of the treatments (P>0.05) as it was recorded from 62.56 to 65.25. Finally, it was found that increasing the concentration of hydrogen peroxide along with the washing cycles could significantly improve the color of waste water obtained from different washing cycles as well as the whiteness of the resulting surimi.
A. Shahabadi, M. Jafari, F. Ememi, Volume 28, Issue 2 (7-2019)
Abstract
The purpose of this study was evaluation of export performance of Iranian caviar and identification of its affecting factors. Therefore, this study has examined theimpact of variables like ability of strategic sustainability management, the measure of export market-oriented and export flexibility taking into consideration of environmental differences between internal and external market on export performance of Iran's caviar. Current research with the view to objective is applied research and with a view to data collection is descriptive research and Statistical population of this study to include all managers of exporting firms Caviar exporting companies in Iran. Cronbach's alpha was 0.9 which indicates that the reliability of the questionnaire is appropriate. Connection between research variable has been investigated through path analysis in LISREL Version 8.8 software. The consequence of this study shows that strategic sustainability management has the most impact on Caviar export performance of Iran. After that parameters of export flexibility, export market-oriented behavior finally dynamic of market have significant and positive impact on export performance .
Mona Hasankhani, Aliakbar Hedayati, Mohammad Mazandarani, Tahere Bagheri, Ali Jafar Nodeh, Volume 29, Issue 3 (8-2020)
Abstract
The objective of this study was to investigate the interaction of salinity levels on the characteristics of mucus and serum indices of common carp (Cyprinus carpio) in exposure to chlorpyrifos pesticide. 250 common carp with an average weight of 20 g were distributed in four treatments each with three replicates including: salinity 0, 4, 8 and 12 ppt and were distributed for 7 days. Then one group was placed for 4 days in exposure to pesticide with an acute concentration of 150 ppm and the second group was placed for 7 days in the with sub-acute concentration of 15 ppm. The results showed that salinity stress has lethal effects with severe and adverse physiological toxicity on common carp. The results of a study of mucus and serum biochemical indices showed that ALT enzyme had significant effect on serum ALT, serum and mucus lysosomes, serum albumin and serum glucose (p<0.05). Eventually it was shown that salinity and pesticide stress have a detrimental effect on vital activities, biochemical indices, liver enzymes and immune system of common carp.
Jafar Kamani, Abbasali Motallebi Moghanjughi, Vadoud Razavilar, Nourdahr Rokni, Volume 29, Issue 5 (12-2020)
Abstract
Rainbow trout filet is a high nutritive value product that can be introduced to consumers successfully which have short shelf life. In this study, the effects of Nano-capsulated chitosan analyzed with and without sodium acetate coatings on increasing the shelf life of refrigerated Rainbow trout filets (Onchorynchus mykiss) during refrigerator storage (4±1°C) and in polyethylene with zipper bag during 16 days. For this purpose, the Rainbow trout filets were immersed in five treatments that included: solutions of NCH1%, NCH2%, NCH1%+NaOAC1.5%, NCH2%+ NaOAC1.5% and distilled water solution as a control sample. The antimicrobial effect of Nano-capsulated chitosan coating with and without sodium acetate was evaluated by counting of aerobic mesophilic bacteria, lactic, psychrophilic bacteria and chemical properties including pH, TBA, PV and TVB-N. Sensory evaluation determined by 5 evaluator persons who were asked to evaluate the flavor, odor, color, texture and overall acceptability of samples in days 0, 3, 6, 10, 12. Sensorial, chemical and microbial results evaluated by ANOVA and Duncan tests. Sensorial, chemical and microbial results showed that in end of storage Nano-capsulated chitosan with and without sodium acetate 1.5% were better significantly compared to control group (p<0.05). In this study, the sample contained NCH2%+ NaOAC1.5% coating treatment showed the best results of storage. The results of Sensorial, chemical and microbial tests of Nano-capsulated chitosan and Nano-capsulated chitosan with sodium acetate indicted that shelf life storage were increased from 6 days in control treatment to 12 and 16 days, respectively.
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