|
|
|
|
|
|
|
RSS |

|
|
 |
|
|
|
|
 |
Search published articles |
 |
|
Showing 2 results for Nassiri
G. Attaran Farimani , H.r. Nassiri , M. Rabbaniha , S.a. Mousavi Golsefid, Volume 23, Issue 1 (4-2014)
Abstract
The survey of fish larvae assemblage variation was done in 2013 seasonally at the coastal areas of Southeastern side of Chabahar Bay. Sampling was done in 3 stations at day and night periods by a Bongo-Net with 300μ of mesh size. 29 families were identified. Clupeidae, Gobiidae and Blenniidae were dominant with more than 50% relative abundance. The PCA result was shown there were two separated groups among day and night fish larval assemblages. Blenniidae ،Scombridae and Clupeidae in day times, Clupeidae ،Gobiidae and Sparidae in night times were more dominant among different families. Station 1 had more fish larvae abundance in autumn in nights and days (54.77 and 79.67 larvae per 10 meter). The average of Shannon index was (0.54 ±0.88 and 0.63± 0.97) in days and nights respectively.Significant increase of larval abundance at station 3 in nights could be due to reduced vessel traffic. *Corresponding author
Ghazal Mohamadi Ahvazi, Mohamad Taghi Beigi Nassiri, Mahmood Nazari, Ayah Sadat Sadr, Volume 33, Issue 1 (4-2024)
Abstract
This research was conducted to identify and validate the simple sequence repeats (SSR) markers related to yellowfin barbel (Luciobarbus xanthopterus) using RNA-Seq data. After collecting the liver tissue, RNA was extracted from the samples. The samples were sequenced using the Illumina Novaseq 6000 platform. Trinity software (version 2.15.1) was used to reconstruct transcripts. Identification of SSR was done using the MISA web server. The primers needed in the polymerase chain reaction for 5 SSR markers were designed by PRIMER 3 software and validated using 45 yellowfin barbel. Transcript assembly by the Trinity program generated 941,894 unigenes and 1,768,662 transcripts. In this study, the examination of 1,276,825 sequences by MISA software led to the identification of 349,871 potential SSR markers. Among the SSRs, di- and trinucleotide repeats had the highest number of repetitions with 89.37% and 8.05%, respectively. From the 5 primers used, only two of them showed polymorphism. The expected and observed heterozygosity for the MN1359 locus were calculated as 0.785 and 0.147, and for the GM1371 locus given 0.770 and 0.093, respectively. Moreover, the chi-square test showed that population was not in Hardy-Weinberg equilibrium. The results of different genetic diversity criteria showed a decrease in diversity in yellowfin barbel. Generally, this research showed that the new SSRs can be helpful for molecular breeding, functional genomics studies, and the genetic diversity analysis of yellowfin barbel.
|
|