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Showing 8 results for Antioxidant Enzymes
M. Youneszadeh Fashalami, A.p. Salati, S. Keyvanshokooh,
Volume 26, Issue 4 (11-2017)
Abstract
One of the most important defense systems in fish to keep homeostasis is antioxidant defense system. To assay antioxidant enzymes in ovarian maturation process in cultured Sterlet sturgeon Acipenser ruthenus females, 24 fish were identified in four groups: previtellogenic, vitellogenic, post-vitellogenic and atresia (6 in each group) and blood samples were taken. Maturity stages were identified on the basis of histological criteria and direct observation. The activity of catalase (CAT), superoxide dismutase (SOD(, glutathione peroxidase (GPX) and malondialdehyde (MDA) content in blood was investigated. The results showed CAT and GPX activity decreased in the vitellogenic stage in compare to previtellogenic (P <0.05), but showed an increase till atresia (P <0.05). SOD activity in different stages was not significantly different (P> 0.05). MDA concentration in different groups did not show significant changes (P> 0.05). The results of this study showed nevertheless the metabolism increased in the process of sexual maturation, the antioxidant defense is could effectively protect fish against these changes.
L. Farzollahi, K. Sarvi Moghanlou, A. Imani,
Volume 28, Issue 1 (5-2019)
Abstract
The aims of present study were to investigate the effects of Chicory (Cichorium intybus L.) and Hypericum perforatum extracts on immune indices and antioxidant enzymes activity of rainbow trout (Oncorhynchus mykiss). For this purpose, 720 fish with an average weight of 100±5 g were randomly allocated to four distinct treatments including: a control group, 3% chicory extract, 3% extract of H. perforatum and combined treatment containing 1.5% chicory extract and 1.5% extract of ) H. perforatum). The experiments lasted for 12 weeks. Immune parameters including lysozyme, alternative complement activity and total immunoglobulin content of serum along with the activity of antioxidant enzymes namely catalase, superoxide dismutase and glutathione peroxidase were measured. Our results showed that dietary supplementation of 3% chicory extract significantly increased total immunoglobulin and lysozyme enzyme activity compared to the control group (p<0.05). The highest level of alternative complement activity was observed in simultaneous supplementation of 1.5% chicory and 1.5% H. perforatum extract (p<0.05). Also, single and combined dietary inclusion of both herbal extracts resulted in increased SOD and GPX enzymes activity in comparison to control group received diet devoid of any herbal extract (p<0.05). In addition, the highest and lowest activity of catalase was observed in the control group and 3% H. perforatum, respectively (p<0.05). In conclusion, dietary inclusion of 3% Chicory extract would improve the immune indices and including 3% H. perforatum extract increased the antioxidant enzymes activity of rainbow trout.
P. Akbary, F. Debashi, R. Fadai Raeini,
Volume 28, Issue 3 (9-2019)
Abstract
The objective of the study was to examine the effects of red seaweed Jania adhaerens J.V. Lamouroux extract (JE) on blood biochemical parameters and antioxidant enzyme activity of grey mullet, Mugil cephalus, after 60 days. Four experimental diets were prepared: JE0 as a control group; JE5, JE10 and JE 15, which included 0, 5, 10 and 15 mg JE kg-1 diet respectively. One hundred twenty grey mullet weighting 14.95± 2.01 g (mean± SE) was randomly divided into four groups corresponding to the different feeding regimes.After 60 days feeding with above diets, No significant differences were evident in albumin and catalase (CAT) between the control group and treatments (P>0.05). The lowest levels of glucose (GLU), cholesterol (CHO), alkaline phosphatase (ALP), alanine aminotransferase (ALT) and the highest glutathione (GSH (activity were shown in JE15. Treatments containing different levels of JE showed significantly different in superoxide dismutase (SOD), MDA, GSH, total protein and globulin compared with control treatment (P<0.05). Overall, the results of the experiment revealed that grey mullet fed with JE10 and JE15 diets showed improvement of the activity of antioxidant enzymes and blood biochemical parameters and use of diet containing 15 mg/kg J.adhaerens extract could suggest for grey mullet.
E. Ahmadifar, H. Adineh, R. Fadaee Raienee, S. Moghaddam Far,
Volume 28, Issue 3 (9-2019)
Abstract
The present study was conducted to determine the effect of saffron petal powder on the growth and activity of antioxidant enzymes in the grass carp larva (Ctenopharyngodon idella). Fish larva (0.75 ± 0.03 g and 4.39± 0.09 cm) were fed separately with four levels of saffron petal powder diets which were included; P0 (0%), P1 (2%), P2 (4%) and P3 (6%) for 60 days. One thousand two hundred fish were divided randomly into 12 tanks (4 experimental treatments and each with 3 replications). End of the experiment, growth (final weight, final length, daily growth rate, weight gain, specific growth rate and condition factor), feed (food conversion ratio and food conversion efficiency) and Antioxidant (glutathione peroxidase, superoxide dismutase, malonyldialdehyde and catalase) parameters were evaluated. Growth parameters were enhanced following feeding with saffron petal powder compared with the control group. The final weight and specific growth rate in P2 treatment increased significantly compared to control, while there was a significant reduction in food conversion ratio (FCR) in this treatment. In addition, the activities of MDA and Catalase were significantly decreased in the fish larvae fed with different levels of supplement compared to those of the control group. A significant enhancement in the SOD and GPX parameters of P3 compared to control. This research demonstrated that the saffron petal powder of 6% could be used as a promising feed additive in aquaculture.
Matin Shakoori, Mansour Rezaei, Yadolah Chashnidel, Reza Safari, Hamed Gholipour,
Volume 29, Issue 2 (6-2020)
Abstract
This study was done to investigate the effect of microencapsulated and non-encapsulated spirulina (Spirulina platensis) powder on the antioxidant enzymes activity and nutrient digestibility of broiler chicks for 6 weeks. A total of 360 one-day old broiler chicks (male) Ross 308 strain was divided into 9 treatments, 4 replicates (10 chicks in each replicate) in a completely randomized design. Experimental treatments include: basal diet without any additives (negative control), basal diet + antibiotic (positive control), 1-basal diet + vitamin E, 2-basal diet + 0.33% spirulina microencapsulated, 3-basal diet + 0.66% spirulina microencapsulated, 4-basal diet + 1% spirulina microencapsulated, 5-basal diet + 0.33% non-encapsulated spirulina, 6-basal diet + 0.66% non-encapsulated spirulina and 7-basal diet + 1% non-encapsulated spirulina. Results showed that the blood super oxide dismutase activity increased in chicks fed with encapsulated spirulina powder and non-encapsulated spirulina powder at the level of 1 percent (p<0.05). But the activity of catalase and glutathione peroxidase enzymes did not show any significant difference (p>0.05). The effect of experimental treatments on nutrient digestibility showed that no significant different experimental groups (p>0.05). The addition of spirulina powder and encapsulated spirulina powder increased antioxidant activity in the diet of Ross 308 broiler chicks.
Rahimollah Bakhtiari, Kourosh Sarvi Moghanlou, Behrooz Atashbar Kangarloei, Ahmad Imani, Mojtaba Pourahad Anzabi,
Volume 32, Issue 1 (4-2023)
Abstract
Regarding the use of cypermethrin insecticide in agriculture in West Azerbaijan and the possibility of its entry into the Urmia Lake, the present study was conducted to investigate the effect of cypermethrin on growth, survival, and some physiological parameters of Artemia urmiana. For this purpose, the LC50 of cypermethrin for 24, 48, and 96 hours in the nauplii, post-larva, and adult stages were obtained using the probit test. The average LC50 of 96 hours was used to study the effect of chronic toxicity. Then, the Naplii was cultured in 4 treatments including the control group, 25% LC50, 50% LC50, and 100% LC50 for 15 days. At the end of the experiment, growth, survival, antioxidant enzymes activity, and digestive enzymes activity of the whole body were evaluated. The results showed that different concentrations of cypermethrin decreased the growth and survival of A. urmiana (p<0.05) and the amount of this decrease depended on the age of Artemia and the exposure duration. The highest decrease in growth and survival of different life stages of A. urmiana was observed in the 100% LC50 group. The results of antioxidant enzymes activity showed that cypermethrin decreased the levels of superoxide dismutase, catalase, and malondialdehyde (p<0.05), but there was no change in the activity of glutathione peroxidase (p>0.05). Also, the activities of lipase and alpha-amylase enzymes were decreased (p<0.05), but no significant difference was observed in the alkaline protease activity (p>0.05). In conclusion, the cypermethrin insecticide decreased the growth, survival, and antioxidant and digestive enzymes activities in A. urmiana.
Eisa Falahat Naserabad, Esmaeil Kazemi, Seyed Abdolhamid Hoseini, Mohamad Meisam Salahi Ardakani, Roghieh Mahmoudi,
Volume 32, Issue 3 (9-2023)
Abstract
In this research, the effect of selenium nanoparticles on the performance of antioxidant enzymes, including superoxide dismutar (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), and catalase (CAT) levels, and male reproductive egg ions of rainbow trout was investigated. The number of 144 fertile pieces of 4-year-old Iranian females was divided into four treatments in three replications. The treatments were fed with diet containing zero (control), 0.5, 1 and 2 mg of selenium nanoparticles per kg of diet for 60 days until egg maturation. Based on the results of the research, the values of SOD, CAT, and MDA have shown significant differences in the eggs from different treatments (p<0.05). The lowest level of SOD enzyme and the highest level of CAT enzyme were observed in the control treatment, which showed a significant difference with other treatments. There was no significant difference in the amount of GPx enzyme between different treatments (p>05). The amount of MDA enzyme showed a significant difference among the treatments, while the control group had the highest level of 363±16.8 mmol/ml and the fish fed with selenium nanoparticles at 2 mg/kg had the lowest level of 181±9.5 mmol/ml Comparing the average amounts of magnesium and calcium among different treatments showed a significant increase in the amounts of the mentioned ions in the treatment fed with selenium nanoparticles at the rate of 2 mg/kg of food compared to other treatments (p<0.05). In general, considering the decreasing role of antioxidant enzymes and its increasing role in the amounts of magnesium and calcium inorganic compounds in the eggs of mothers fed with more selenium nanoparticles, this essential micronutrient can be used as It should be considered as a suitable candidate for further research in order to improve the quality of spawning eggs of female rainbow trout by adding to the diet.
Fereshteh Hemmat Koohsar, Javad Mirdar Harijani, Ahmad Gharaei,
Volume 32, Issue 6 (2-2024)
Abstract
In this study, the effect of different levels of vitamin C on growth indices, hematology, and antioxidant enzymes activity was studied in Schizothorax zarudnyi. For this purpose, a total of 144 fish was randomly distributed in 12 fiberglass tanks (12 fish per tanks) and fed with 3 dietary treatments containing 100, 500 and 1000 mg/kg of vitamin C and a control group without vitamin C for 60 days. The biological parameters of juveniles were measured at the end of the experimental period. To measure liver enzymes, antioxidant enzymes and also blood parameters, blood sampling from the tail vein of fish were performed. The results of growth indices in different treatments did not show a significant difference with the control group (p<0.05). The highest mean final weight, weight gain and specific growth rate, condition factor, body weight gain percentage and lowest feed conversion ratio were observed in 1000 mg/kg vitamin C treatment. The amount of red blood cells in all treatments was significantly different from the control treatment (p<0.05). The highest amount of red blood cells was in the treatment of 1000 mg/kg of vitamin C, which was significantly different from the control group (p <0.05). Also, white blood cell count increased in all treatments and there was a significant difference with the control group (p<0.05). The highest amount of white blood cells was recorded in the treatment of 500 mg/kg of vitamin C. Which had a significant difference with the control group (p<0.05). The alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase had a significant difference with the control group (p<0.05). The antioxidant enzymes catalase, glutathione reductase, glutathione peroxidase, superoxide dismutase, total immunoglobulin, and total protein are significantly different from the control group (p<0.05). According to the analysis of the results, it can be acknowledged that vitamin C has a positive effect on blood factors and antioxidant enzymes in Schizothorax zarudnyi.
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