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In the present study, sodium hypochlorite with a concentration 0.01% for 5 minutes was used as a new technique to de-adhesion of fertilized egg of Siberian sturgeon (Acipenser baerii) and its efficiency was compared with clay solution. The lowest amount of dead and fungal eggs (37.78±2.10%) and the highest fertilization (69.35±1.75%) and hatching rates (62.21±2.10%) were observed in eggs treated with sodium hypochlorite (p<0.05). Moreover, the highest weight (13.03±0.23 mg) and length (11.82±0.17 mm) of larvae were found in the de-adhesion treatment with sodium hypochlorite which had a significant difference with clay treatment (p<0.05). There was a significant difference in the incubation time between treatments (p<0.05), so that the de-adhesion treatment with clay had the highest incubation time (2093.75±21.62 degree-hour). The results of this study demonstrated that using sodium hypochlorite with a concentration of 0.01% is a simple, cheap and fast method for egg de-adhesion of Siberian sturgeon.