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Showing 2 results for Bioassay
M Mehrabi, M Soltani, H Ebrahimzade, S.s Mirzargar, E Sharifpoor, S Ghasemi, A Dashtiannassab, B Ghaednia,
Volume 19, Issue 1 (4-2010)
Abstract
Silver ion and hydrogen peroxide act synergistically as a strong disinfectant. The purpose of this study was to assess the effect of combined hydrogen peroxide 50% and silver ion 0.05% at four life stages of Indian white shrimp (Fenneropenaeus indicus) and to evaluate the feasibility of using this substance in shrimp culture. The Trimmed Spearman-karber software was applied for determining ECso/96h and LC5o/96h with 95% confidence limit on the 6000 shrimp based on OECD static method.
The experiments were conducted in Iran Shrimp Research Center, Bushehr, during 2005 -2006. The
ECso values of 132.5, 67.89, 55.56, 51.95 and 48.6ppm were obtained in PL 15 stage after 12, 24, 48, 72
and 96 hours, respectively. Also these were 147.57, 70.83, 60.01, 54.89, 41.19 for PL45 stage, and
306.43, 174.14, 113.62, 78.21, 61.96 for sub adult stage (12±1 grams), respectively. In addition, the EC5o values of243.25, 130.55, 75.56, 61.18 and 51.59ppm were obtained at adult stage (20±2 grams), respectively. The LC50 values of239.81, 101, 74.28, 65.72 and 61.45ppm were obtained in PL 15 stage
after 12, 24, 48, 72 and 96 hours, respectively. Also these were 304.56, 160.12, 113.1, 93.69, 79.38 for
PL4s stage, and 712.13, 518.44, 265.29, 145.53, 103.76 for sub adult stage, respectively. In addition, the LC5o values of 827.75, 508.91, 317.3, 139.44 and 85.88ppm were obtained at adult stage, respectively. The statistical results showed that the "no observed effect concentration" (NOEC) of this substance was 20ppm, and the "lowest observed effect concentration" (LOEC) was 40ppm, thus "maximum allowable concentration" (MAC) value was determined 28.8ppm on the Indian white shrimp. Therefore this combined chemical should be used under determined MAC value with a complete precautionary as a disinfectant for Indian white shrimp.
Mohammad Akhavan-Bahabadi, Hamed Paknejad, Aliakbar Hedayati, Mehran Habibi-Rezaei3,
Volume 32, Issue 3 (9-2023)
Abstract
Fish epidermal mucus protein extraction for bioassay-guided screening is challenging and variable. In this study, four different protein extraction methods, including acetone, trichloroacetic acid (TCA)-acetone, methanol, and solid-phase extraction (SPE), were investigated to study their efficiency in ultrafiltration and to obtain the appropriate peaks in HPLC to measure biological activities. The presented data shows that the solid phase extraction method is better than the other three tested methods in quantity (total protein yield) and quality (protein profile observed in SDS-PAGE) of proteins and peptides from the epidermal mucus of Caspian monkey goby. On the other hand, due to the epidermal mucus hydrophobic nature, all three extraction methods including acetone, (TCA)-acetone and methanol led to the irreversible proteins aggregation, makes ultrafiltration, HPLC, and consequently bioactivity screening impossible. In this study, the highest amount of protein (4.676 μg/ml) (P<0.01) and the best protein profile (13 bands) were related to solid phase extraction. Also, this method simultaneously leads to the extraction, concentration, and initial purification of the samples and therefore improves the efficiency ultrafiltration and HPLC. Thus, we introduce this method for the isolation of active proteins and peptides from various complex biological samples, including fish epidermal mucus.
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