Omidvar Farhadian, Maryam Hematian, Safiollah Heidari,
Volume 33, Issue 3 (8-2024)
Abstract
In the world of aquaculture, microalgae are mainly used as a source of extracting pigments or producing live food for aquatic animals. This research aimed to investigate the combined effects of photoperiods and salinity on the growth, reproduction, and pigments of marine microalgae Tetraselmis tetrathele. The experiment was carried out as a completely randomized design at three photoperiods (3L light: 3D dark, 4L:4D, and 12L:12D) and five different salinities (20, 25, 30, 35, and 40 g/L) with three replicates in each treatment for 23 days. Results showed that the increase in photoperiod and salinity significantly (p<0.05) increased density, specific growth rate, and biomass. The highest cell density and specific growth rate were 11.2 × 107 cells/mL and 0.206 per day, respectively, in the 3L:3D light regime and 35 g/L salinity. The maximum dry biomass and total carotenoids were 1.4 and 4.6 mg/L at 4L:4D and 40 g/L, respectively. Maximum chlorophyll a and b were 22.3 and 35.4mg/L at 3L:3D and 25 g/L salinity, respectively. In conclusion, this study indicated that 3L:3D and 4L:4D photoperiod combined with a high salinity could be improved growth and biomass as well as relative increase in the pigment contents.
