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Showing 2 results for Ethanolic Extract
S. Bahroodi, M. A. Nematollahi, M. R. Aghasadeghi, M. Nazemi, B. Behroz, Volume 23, Issue 3 (10-2014)
Abstract
In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells.
A. Dashtiannasab, M. Mesbah, R. Peyghan, S. Kakoolaki, Volume 23, Issue 3 (10-2014)
Abstract
In this study, the effect of ethanolic extracts of Sargassum angustifolium on growth and survival of shrimp Litopenaeus vannamei juvenile was investigated under challenge with shrimp pathogen bacteria Vibrio harveyi. Powder form of the extract was bioencapsulated in Artemia and fed to L. vannamei juvenile reared as 5 groups inclouding C- (unenriched Artemia, without bacteria), C+ (unenriched Artemia, with bacteria), T1 (enriched Artemia with 200 mg l-1 SA extract, with bacteria), T2 (enriched Artemia with 400 mg l-1 SA extract, with bacteria), T3 (enriched Artemia with 600 mg l-1 SA extract, with bacteria). One week after culture all groups except C- were inoculated with V. harveyi at the rate of 1.5 × 108 CFU ml-1 for 15 minutes then after every water exchange 10 ml of V. harveyi at the rate of 1.5 × 107 CFU ml-1 was added to aquaria. Shrimps at group C- showed maximum survival (86.6%), specific growth rate (SGR, 11.33%) and less bacterial load (0.5 ± 0.03× 102 CFU g-1 tissue). While (C2) exhibited lowest survival (33.3%), SGR (9.90%) and more bacterial load (3.4 ± 0.05× 105 CFU g-1 tissue) and the difference was significant (p<0.05). In treatment groups survival and SGR were significantly (p<0.05) more than C+ and less than C-, also bacterial load were less than C+ and more than C-. Among treatment groups T2 that fed with enriched artemia with 400 mg l-1 SA extract gave better results
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