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Showing 2 results for Genetic Variation
S. Eagderi, A. Jouladeh-Roudbar, J. Imani Harsini, M. Rostami,
Volume 28, Issue 3 (9-2019)
Abstract
Up to now, twelve species of the genus Alburnoides belonged to Cyprinidae have been described from Iranian inland water solely based on the morphological characteristics. The described key characters for these species have no efficiency for their discrimination, and cannot help their precise diagnosis. Therefore, this study was conducted to study the phylogenetic relationship and verification of these species based on COI gene. For this purpose, 70 specimens of Alburnoides species were collected from 20 sampling sites in six Iranian inland water basins. To determination of inter- and intra-species genetic differences, the DNA of sampled specimens were extracted based on Phenol-chloroform method, their COI gene were amplified using thermal cycler and were sequenced after purifications. Based on the results, the members of the Iranian species of the genus Alburnoides were divided into seven linages. Maximum and minimum intra-species genetic distances based on K2P criterion were found between the populations of A. samiii, and A. coadi, A. holciki, A. parhami and A. nicolausi, respectively. Furthermore, maximum and minimum inter-species genetic distances based on K2P criterion were calculated between A. qanati and A. holciki (7.56), and A. parhami and A. holciki (0.33). Based on the results of this study using COI gene, the validity of A. parhami, A. coadi and A. nicolausi were not confirmed and it is suggested to be synonym of A. holciki, A. namaki and A. idignensis, respectively.
Sajad Nazari, Mohammad Pourkazemi, Majidreza Khoshkholgh,
Volume 29, Issue 1 (3-2020)
Abstract
Direct mitochondrial DNA (mtDNA) control region sequencing analysis was used to investigate population genetic structure of Persian sturgeon (Acipenser persicus) in the Caspian Sea. A total of 45 specimens were collected from different locations of the Caspian Sea. mtDNA control region was amplified using PCR. Direct sequencing was performed according to a standard method. The results showed that 12 haplotypes were observed among 45 samples in the method. The highest numbers of haplotypes were observed in the Sefidrud River in which 3 haplotypes A, B and E among them were specific for the river and were not observed in the other locations. The average haplotype diversity (h) and nucleotide diversity (π) were 0.795±0.037 and 0.0062±0.0046, for control region sequencing, respectively. The results of Fst based on kimura- 2 parameters method and analysis of molecular variance (AMOVA) demonstrated that most variations occurred among the samples, and between samples of the Sefidrud and Russia and Azerbaijan are statistically significant (P<0.0001). Therefore, three distinct populations including Sefidrud, Russia and Azerbaijan were identified. As mtDNA control region is a highly variable segment, it may be used as a potential marker for identifying populations and for determining their management and conservation units, leading to the useful application of molecular markers in investigating conservation genetics of the Persian sturgeon.
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