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Showing 2 results for Total Protein
M. Afsharnasb, Z. Shojaei, A. Dashtyannasab,
Volume 23, Issue 1 (4-2014)
Abstract
The investigation of Total Hemocyte Count (THC) and Total Protein Plasma (TPP) in shrimp juvenile Litopenaeus vannamei that exposed with Monodon Baculovirus (MBV) was carried out. Three hundred and sixty shrimps with average weight 7 to 12 g were selected from Heleh site in Bousher province and transported to Iran Shrimp Research Institute. The shrimp was acclimated during two days for stress reduction and adaptation. The shrimps were cheeked by PCR for WSSV, MBV, HPV, TSV, IHHNV and NHP and the result showed they are free of these diseases. The experiment was designed with selected 60 shrimps in triplicate as treatment and exposed with MBV during 12 hours and control group with triplicate without exposing with MBV. The hemolymph was withdrawn from abdominal segments of samples for measuring THC and TPC evaluation at designed hours (0, 12, 24, 48, 72 and 96). The PCR test confirmed the MBV in treatment and the control were free of viruses. The results showed that the difference of THC and TPP value between treatment and control group during the experiment was significant (P < 0.05). This finding can be used for assessing the health of shrimp culture and prevention of MBV.
Mohammad Binaii, Abolfaz Sepahdari, Maryam Ghiasi, Alireza Babaalian Amiri, Hamzehali Mattani, Farshideh Habibi,
Volume 31, Issue 4 (11-2022)
Abstract
In this study, dry leaf powder of nettle and dwarf elder in two combinations and three treatments including treatment 1 (dwarf elder 50 g + nettle 60 g/ kg diet), treatment 2 (dwarf elder 50 g + nettle 120 g g/ kg diet) and control (normal diet) were used and 180 carp (52.64 ± 2.74 g) were fed during 8 weeks. The blood samples were taken in the 4 and 8 week of the trial. At the end of week 8, the fish were challenged to Aeromonas hydrophila. The results showed that the final weight, body weight gain and specific growth rate in treatment 2 and the food conversion factor of this treatment significantly increased and decreased, respectively, compared to the control. The levels of MCV, MCH, MCHC, glucose, cholesterol, albumin, alanine aminotransferase and aspartate aminotransferase were not shown any significant difference between the treatments and the control. In the 4th and 8th week, the amount of red blood cells, hematocrit and hemoglobin of the treatments were significantly higher than the control. At the end of week 4, the amount of white blood cells was significantly different between treatment 2 and the control, but at the end of week 8, this index increased significantly in the treatments compared to the control. The percentage of neutrophil cells, total protein and total IgM in the treatments in weeks 4 and 8 were increased significantly compared to the control. The amount of triglycerides of the treatments in the 8th week had a significant decrease compared to the control. During the 4th and 8th week, the lysozyme activity of the treatments increased significantly compared to the control, and the highest activity was in treatment 2. In week 4, the oxygen free radical production of treatment 2 had a significant increase compared to the control, but in week 8, a significant increase were seen in the treatments compared to the control. The percentages of survival after challenging to Aeromonas hydrophila were 71.6%, 69.9% and 22.6% in the treatment 1, 2 and control, respectively. The results of this study showed that using the combination of nettle and dwarf elder in both treatments can improve the growth and safety of carp fish, but treatment 2 had better results.
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