Iranian Scientific Fisheries Journal
مجله علمي شيلات ايران
isfj
Agriculture
http://isfj.ir
1
admin
1026-1354
2322-5998
10.18869/acadpub.isfj
000000
000000
000000
fa
jalali
1396
8
1
gregorian
2017
11
1
26
4
online
1
fulltext
fa
جداسازی و شناسایی سیست Dunaliella salina (Chlorophyceae) از رسوبات ساحلی لیپار (دریای عمان) با استفاده از آنالیز مولکولی
Isolation and molecular identification of Dunaliella salina (Chlorophyceae) cyst from sediments of the Lipar coast (Oman Sea)
اكولوژي محيطهاي آبي
اكولوژي محيطهاي آبي
پژوهشي
Research
<p dir="RTL" style="text-align: justify;"><span style="font-family:b compset;"><span style="font-size:12.0pt;">برخی از گونه­های فیتوپلانکتون</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">­در</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">شرایط</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">نامساعد</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">و در چرخه زندگی تولیدمثلی خود سیست تولید</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">کرده که در رسوبات</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">کف ته­نشین</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">می</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">­</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">شوند</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">و تحت</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">شرایط</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">مساعد</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">مجدداً شکوفا شده</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">و به</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">ستون</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">آب</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">برمی­گردند</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> بمنظور شناسایی دقیق مورفولوژی،</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">گونه­های</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">فیتوپلانکتون­</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">دو</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">مرحله</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">ی</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">سیست</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">و متحرک</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">بررسی</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">گردیدند</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">زیرا</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">برخی</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">سیست­های</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">مشابه</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">فیتوپلانکتون­</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">متفاوتی</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">تبدیل</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">می­شوند</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> هدف</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">این</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">تحقیق،</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">کشت</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">سیست­های</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">موجود</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">رسوبات</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> خلیج چابهار</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">منطقه</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">لیپار و شناسایی</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">نمونه</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">شکوفا</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">شده</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">میباشد</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">رسوبات</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> از منطقه لیپار در سال 1394</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">توسط </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Ekman Grab</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> با سطح</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">جمع کنندگی </span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">225 </span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">سانتیمتر</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">مربع جمعآوری</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">گردید.</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">سیست زنده گرد ناشناخته بصورت انفرادی از رسوبات جدا­سازی شد و</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">در فایکولب در شرایط کاملاً</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">استریل</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">بصورت</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">جداگانه تحت</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">شرایط 12 ساعت روشنایی و 12 ساعت تاریکی در درجه حرارت </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">°C</span></span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">1</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">±</span></span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">25</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">در محیط کشت </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">F<sub>2</sub></span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">،</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> کشت داده شدند.</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">بررسی مورفولوژی گونه شکوفا شده نشان داد که سیست جدا شده متعلق به گونه </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">Dunaliella salina</span></span></em><span style="font-family:b compset;"><span style="font-size:12.0pt;"> میباشد. جهت تائید شناسایی</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">استرین خالص حاصل از کشت سیست مورد­نظر</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">،</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">استخراج</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;">DNA</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">و سپس واکنش زنجیره­ای پلی­مراز </span></span><span style="font-family:b compset;">(</span><span dir="LTR"><span style="font-family:times new roman,serif;">PCR</span></span><span style="font-family:b compset;">)</span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> انجام</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> گردید</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> توالی ژنی گونه </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">D. salina</span></span></em><span style="font-family:b compset;"><span style="font-size:12.0pt;"> با توالی ژنی گونه­های مشابه از بانک ژن مقایسه گردید. بررسی مولکولی و فیلوژنی گونه نشان­ داد که گونه</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">شکوفا</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">شده</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">از نظر توالی نوکلئوتیدی بیشترین</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">شباهت را با گونه </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">D. salina</span></span></em> <span style="font-family:b compset;"><span style="font-size:12.0pt;">دارد.</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;"> همچنین</span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">، این مطالعه نشان داد که گونه </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">D. salina</span></span></em> <span style="font-family:b compset;"><span style="font-size:12.0pt;">از سواحل جنوب ایران </span></span><span style="font-family:b compset;"><span style="font-size:12.0pt;">توانایی</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">تولید</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">سیست</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">را</span></span> <span style="font-family:b compset;"><span style="font-size:12.0pt;">دارد</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span><br>
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</p>
<p> Some species of phytoplankton under unfavorable environmental conditions and in their sexual life cycle produce resting cyst that settle to the bottom sediments and become motile in the water column when suitable conditions provided. Phytoplankton species were studied in two stages of motile and cyst for accurate identification of morphology, because some similar cysts may convert to a different phytoplankton and vice versa. The objectives of this study were to culture cysts that isolated from sediments of Lipar zone located in the southeast coast of Iran and to identify excysted samples. Sediment samples from Lipar zone were collected by Ekman grab with 225 cm<sup>2</sup> area in 2015. Unknown live and orbicular single cysts were isolated from sediments and cultured in sterile conditions in phycolab using f<sub>2</sub> medium under 12 hours light and 12 hours dark cycles at 25±1°C. Assessment of excysted plankton morphology revealed that the cyst is belonged to <em>Dunaliella salina </em>species. In order to confirm this morphological identification, DNA of the plankton was extracted and PCR was performed. The sequence of the sample was compared with the sequences of the similar species from GenBank. Phylogeny and molecular analysis confirmed this identification. The present study revealed that <em>D. salina</em> from the south coast of Iran was able to produce resting cyst.<br>
</p>
Dunaliella salina, سیست, فیلوژنی, رسوبات, سواحل جنوب ایران
Dunaliella salina, Cyst, Phylogeny, Sediment, South coast of Iran
47
55
http://isfj.ir/browse.php?a_code=A-10-497-6&slc_lang=fa&sid=1
G.
Attaran
گیلان
عطاران فریمان
gilan.attaran@gmail.com
100319475328460022876
100319475328460022876
Yes
Chabahar Maritime University, Faculty of Marine Sciences
دانشگاه دریانوردی و علوم دریایی چابهار
P.
Sadeghi
پروین
صادقی
parvin.sadeghi@gmail.com
100319475328460022877
100319475328460022877
No
دانشگاه دریانوردی و علوم دریایی چابهار
R.
Shirzaii
رقیه
شیرزایی
roghayeshirzaii@yahoo.com
100319475328460022878
100319475328460022878
No
دانشگاه دریانوردی و علوم دریایی چابهار