Iranian Scientific Fisheries Journal
مجله علمي شيلات ايران
isfj
Agriculture
http://isfj.ir
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10.18869/acadpub.isfj
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2022
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مقاله علمی – پژوهشی: فعالیت آنزیم کیموتریپسین استخراج شده از روده فیل ماهی (Huso huso) تحت تأثیر برخی عوامل فیزیکی و شیمیایی
Chymotrypsin activity extracted from beluga (Huso huso) intestine under some physical and chemical factors
فيزيولوژي آبزيان
فيزيولوژي آبزيان
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">در مطالعه حاضر، عوامل فیزیکی و شیمیایی شامل تعیین دما و </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">pH</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> بهینه و اثر </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">بازدارندههای آنزیمی، یونهای فلزی، سورفاکتانتها و عوامل اکسیدکننده<b> </b>بر میزان فعالیت آنزیم کیموتریپسین استخراج شده از روده فیل ماهی </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">(</span></span><i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Huso huso</span></span></i><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">)</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">بررسی گردید. براساس یافتههای این تحقیق، دما و </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">pH</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> بهینه فعالیت آنزیم کیموتریپسین بهترتیب</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">50</span></span></span><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">درجه سانتیگراد</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> و 8 بود و در دمای بالاتر از 50<i> </i></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">درجه سانتیگراد</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> فعالیت آن بهشدت کاهش یافت</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">بازدارندهها شامل </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">SBTI</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">TLCK</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">،</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">PMSF</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">،</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">پارا آمینوبنزآمیدین،</span></span></span> <span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">TPCK</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">،</span></span></span> <span class="MsoFootnoteReference" style="vertical-align:super"><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">، </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">یدواستیک اسید و</span></span></span> <span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">EDTA</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> بطور معنیداری از فعالیت آنزیم جلوگیری کردند که </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">بازدارندههای </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">PMSF</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">SBTI</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">TPCK</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> اثر مهارکنندگی کاملی بر فعالیت آنزیم داشتند</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">(05/0></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">). یونهای </span></span></span><sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">+</span></span></sup><i> </i><sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">+</span></span></sup><sub> </sub><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">سبب افزایش معنیدار و</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> یونهای</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Cu</span></span><sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">2+</span></span></sup><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">،</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Ba</span></span><sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">2+</span></span></sup><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">، </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Co</span></span><sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">2+</span></span></sup><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">،</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Zn<sup>2+</sup></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">،</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Fe</span></span><sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">2+</span></span></sup> <sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">+</span></span></sup><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> باعث کاهش معنیدار فعالیت آنزیم گردیدند (05/0></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">). یونهای</span></span></span><sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">+</span></span></sup><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> و</span></span></span> <span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Na<sup>+</sup></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">اثر معنیداری بر فعالیت آنزیم نشان ندادند (05/0</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">P></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">). فعالیت آنزیم کیموتریپسین در حضور سورفاکتانتهای ساپونین و سدیم کولات </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">افزایش معنیدار (05/0></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">) و در حضور سدیم دودسیل سولفات و عوامل اکسیدکننده (سدیم پربورات و پراکسید هیدروژن) کاهش معنیداری (05/0></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">) نشان داد. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">نتایج مطالعه حاضر نشان داد فعالیت آنزیم کیموتریپسین در روده فیلماهی در حضور بازدارندهها، یونهای فلزی (غیر </span></span></span><sup><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">+</span></span></sup><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">،</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Mg<sup>2+</sup></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">،<sub> </sub></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">K<sup>+</sup></span></span><span style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black"> و</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Na<sup>+</sup></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">)</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">، سدیم </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">دودسیل سولفات، سدیم پربورات و پراکسید هیدروژن کاهش معنیدار و در حضور </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Ca<sup>2+</sup></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">و</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times=""><span style="color:black">Mg<sup>2+</sup></span></span><sub><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">، </span></span></span></sub><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">ساپونین و سدیم کولات </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">افزایش معنیداری داشت </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"><span style="color:black">که میتواند در ارزیابی اثرگذاری آنها بر فعالیت بهینه این آنزیم در فیزیولوژی گوارش فیل ماهی موثر باشد.</span></span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:10pt"><span style="line-height:17.0pt"><span style="font-family:F_Nazanin"><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">In the present study, the physical and chemical factors including optimum temperature and pH, the effect of inhibitors, metal ions, oxidizing agents</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">,</span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> and surfactants were assayed on the chymotrypsin activity from beluga (<i>Huso huso</i>) intestine. According to the obtained results, </span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">the </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">optimum temperature and pH of the chymotrypsin were recorded at 50°C and 8, respectively, and its activity was extremely decreased at temperature>50°C. Inhibitors including SBTI, TLCK, PMSF, </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ρ-aminobenzamidine</span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">, TPCK, pepstatin A, iodoacetic acid and EDTA </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">significantly </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">inhibited the enzyme activity that PMSF, </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">SBTI and TPCK </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">had a completely inhibition effect on the enzymatic </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">activity (P<0.05). The enzyme activity was significantly increased in the presence of Ca<sup>+2</sup> and Mg<sup>+2</sup> and </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">decreased by </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Cu<sup>+2</sup>, Ba<sup>+2</sup>, Co<sup>+2</sup>, Zn<sup>+2</sup>, Fe<sup>+2</sup> and Al<sup>+3</sup> (P<0.05). Na</span></span></span><sup><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">+ </span></span></span></sup><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">and K</span></span></span><sup><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">+</span></span></span></sup><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> did not </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">show any significant effect on the activity of enzyme (P<i>></i>0.05). The enzymatic activity was significantly increased in presence of surfactants including saponin and sodium cholate and showed a significant decrease in presence of SDS and oxidizing agents like sodium perborate and </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">hydrogen peroxide </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(P<0.05). </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Therefore, the results of this study showed that the chymotrypsin activity from beluga intestine had a </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">significant decrease in presence of inhibitors, metal ions (except for Ca<sup>+2</sup>, Mg<sup>+2</sup>, K</span></span></span><sup><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">+</span></span></span></sup><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> and Na</span></span></span><sup><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">+</span></span></span></sup><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">), </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">SDS, sodium perborate and </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">hydrogen peroxide and showed </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">a </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">significant increase in presence of Ca<sup>+2</sup></span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> and</span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Mg<sup>+2</sup></span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">, s</span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">aponin and sodium cholate that it can be </span></span></span><span lang="X-NONE" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">affected the maximum activity of the enzyme in digestive physiology of the beluga.</span></span></span></span></span></span></div>
روده, کیموتریپسین, فیل ماهی, عوامل فیزیکی و شیمیایی
Intestine, Chymotrypsin, Beluga, Physical and chemical factors
17
28
http://isfj.ir/browse.php?a_code=A-10-355-6&slc_lang=fa&sid=1
Abbas
Zamani
عباس
زمانی
a.zamani@malayeru.ac.ir
0322579813
100319475328460034555
Yes
Malayer University
دانشگاه ملایر
Maryam
Khajavi
مریم
خواجوی
marykhajavi@yahoo.com
1842039512
100319475328460034556
No
Malayer University
دانشگاه ملایر