fa مقاله علمی – پژوهشی:‌ تجمع فلزات سنگین جیوه و روی در بافت آبشش و ارتباط آنها با برخی از شاخص‌‌های بیوشیمیایی خون ماهی سی‌‌باس آسیایی (Lates calcarifer) آلودگي محيطهاي آبي آلودگي محيطهاي آبي پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><a name="_Hlk162956121"></a></span></span></span></span><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><a name="_Hlk162956121"><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">این تحقیق با هدف اندازه‌گیری و بررسی ارتباط بین فلزات سنگین (جیوه و روی) با برخی شاخص‌های بیوشیمیایی خونی و بافت</span></span></span></span></a><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black"> آبشش ماهی سی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">باس پرورش یافته در قفس در پاییز 1401 واقع در بندر کنگان انجام گرفته است. نمونه</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">ها پس از تثبیت با الکل، به آزمایشگاه منتقل شدند. شاخص</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">های</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">زیست</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">سنجی</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">ماهیان</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">شامل</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">وزن</span></span></span></span><span dir="LTR" style="color:black">)</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">گرم)</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">به</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">وسیله</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">ترازو</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">و</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">طول</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">کل</span></span></span></span><span dir="LTR" style="color:black">)</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">سانتی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">متر)</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">به</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">وسیله</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">تخته</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">زیست‌سنجی اندازه</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">گیری شدند. سپس از آبشش هر</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">ماهی نمونه تهیه</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">و به‌وسیله دستگاه اسپکتروفتومتری جذب اتمی شعله، میزان غلظت هر فلز به</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">دست آمد. برای ارزیابی تجزیه‌وتحلیل بافتی، نمونه بافت آبشش جداسازی و به‌وسیله دستگاه پاساژ بافت تهیه شده و به</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">روش هموتوکسین ائوزین رنگ‌‌آمیزی شدند. سنجش شاخص‌های بیوشیمیایی خون نیز براساس دستورالعمل کیت تجاری انجام گردید. نتایج این پژوهش نشان داد، میانگین فلز جیوه و روی</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">به</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">ترتیب 1/0 و 5/7 &nbsp;میکروگرم بر گرم در خون ماهی‌ها بود. میانگین غلظت کورتیزول، گلوکز، آلبومین، پروتئین‌‌کل، کلسترول و تری گلیسرید به‌‌ترتیب 5/50 نانوگرم برمیلی‌‌لیتر، 33/154میلی گرم بر دسی‌‌لیتر، 19/1گرم بردسی‌‌لیتر ، 36/3 گرم بر دسی‌‌لیتر ،194 میلی‌‌گرم بر دسی‌‌لیتر و 244 میلی گرم بردسی‌‌لیتر به‌‌دست آمد. در بررسی نمونه بافت آبشش ماهی سی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">باس آسیایی عوارضی مانند پرخونی، خونریزی، هیپرپلازی، نکروز سلولی و تخریب لایه اپیتلیوم رشته‌‌های اولیه و ثانویه آبشش، مشاهده گردید</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">. غلظت فلزات سنگین جیوه و روی در بافت آبشش ماهی سی</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">باس مورد مطالعه، پایین</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">‌‌</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black">تر از حداستاندارد تعیین شده از سوی </span></span></span></span><span dir="LTR" style="color:black">FAO</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black"> و </span></span></span></span><span dir="LTR" style="color:black">WHO</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:80%"><span b="" compset="" style="font-family:"><span style="color:black"> بود.</span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:14.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span><span style="line-height:16.0pt"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">This research was conducted with the aim of measuring and investigating the relationship between heavy metals (mercury and zinc) and some biochemical factors of blood and gill tissue of sea bass (<i>Lates calcarifer</i>) grown in cages in fall of 2022, located in Kangan port.</span></span></span> <a name="_Hlk167090774"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">After fixing the samples with alcohol, they were transferred to the laboratory. The biometric indexes of the fish including weight in grams were measured using a scale and total length in centimeters using a biometric board. Then, a sample of the gills of each piece of fish was prepared and the concentration of each metal was obtained by flame atomic absorption spectrophotometry. To evaluate histological analysis, gill tissue samples were separated and stained by hematoxylin and<b> </b>eosin</span></span></span></a> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">method. Blood biochemical factors were measured according to the instructions of the commercial kit. The results of this research showed that the average metal mercury and zinc were 0.1 and 7.5 microgram</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">/</span></span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">g respectively in the fish blood serum of the samples. which was lower than WHO and FAO standards. The average concentration of cortisol, glucose, albumin, total protein, cholesterol and triglycerides were 50.55 ng/l, 154.33 mg/ dl, 1.19 g/dl, 3.36 g/dl, 194 mg/dl and 244 mg/dl were obtained, respectively. In the examination of the gill tissue sample of Asian seabass, complications such as hyperemia, bleeding, hyperplasia, cell necrosis and destruction of the epithelium layer of the primary and secondary strands of the gill were observed. The concentration of heavy metals mercury and zinc in the gill tissue of the studied seabass was lower than the standard limit determined by FAO and WHO.</span></span></span></span></span></span> سی‌‌باس آسیایی, هیستوپاتولوژی, شاخص‌های بیوشیمیایی, جیوه, روی Asian seabass, Histopathology, Biochemical parameters, Mercury, Zinc 61 75 http://isfj.ir/browse.php?a_code=A-10-646-3&slc_lang=fa&sid=1 Siamak Yousefi Siahkalroodi سیامک یوسفی سیاه کلرودی siamak.yousefi1@gmail.com 100319475328460038558 100319475328460038558 Yes Department of Biology, Faculty of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University گروه زیست شناسی، دانشکده علوم زیستی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی Vahid Morshedi وحید مرشدی morshedi.vahid64@gmail.com 100319475328460038559 100319475328460038559 No Department of Fisheries, Persian Gulf Research Institute, Persian Gulf University گروه شیلات و زیست شناسی دریا، پژوهشکده خلیج فارس، دانشگاه خلیج فارس پرستو محبی درخش mdparastoo1356@yahoo.com 100319475328460038560 100319475328460038560 No Iranian Fisheries Science Research Institute, Agriculture Research Education and Organization (AREEO) موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی مهیار یوسفی سیاه کلرودی mahyaryousefi98@gmail.com 100319475328460038561 100319475328460038561 No Tehran, Iran 4 Faculty of Veterinary Medicine, Science and Research Unit, Islamic Azad University دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، شمری اصیل قاسم محمد shil.bio.jour@gmail.com 100319475328460038562 100319475328460038562 No Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University 5- گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی