per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
1
12
article
Biotechnic of brood stocking, artificial propagation and some physiological indices in farmed Acipenser nudiventris
M. Bahmani
mahmoudbahmani@ymail.com
1
A. Yousefi Jourdehi
2
R. Kazemi
3
M. Pourdehghani
4
A. Hallajian
5
S. Dejhandian
6
J. Jalilpour
7
This study was conducted on farmed Acipenser nudiventris at the International Sturgeon Research Institute from the winter of 2007 till summer 2010. A total of 11 farmed A. nudiventris specimens (7 male and 4 female) were stocked in groups of 4 fish in eight fiberglass tanks (4 tons capacity) on the basis of their sex. Males were fed with a diet with no soybean but containing vitamins. Females were fed with a diet containing soybean and vitamins with 38-40% protein, 13-15% fat, 19.5-20Mj/kg energy, respectively. GnRH was used for artificial propagation of Acipenser nudiventris brood stocks twice for females (dose 10μg/kg with 80:20 ration) and during one occasion for males with a dose of 20 μg/kg. The results of the study indicated that food composition (soybean and vitamins C and E) played a significant and positive role in the reproduction system for females. No significant effect of treatment was observed in Testosterone levels between mature (Mean ±SD: 60.6±8.07ng/ml) and immature (24.42±4.87ng/ml) males, Likewise, no significant differences were found in17α-Hydroxy Progestrone for female (Mean ±SD: 0.106±0.019ng/ml) and for immature fishes (0.031±0.006ng/ml) significant differences was detected in the concentration of Albomine (1.54±0.05mg/dl for females and 1.35±0.07mg/dl for immature females), but there were no significant differences in other biochemical parameters including Glocose, Cortisol, Chlosterole and Triglisride in males and females. There were no significant differences in total weight and length, WBC, RBC, Hb, Hct, MCH, MCHC and MCV parameters in males and females. This study is a step forward towards the development of artificial breeding and rearing sturgeon fish in the country.
http://isfj.ir/article-1-721-en.pdf
Sexual hormones
Reproductive physiology
Sturgeon fish breeding
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
13
22
article
Dietary administration of vitamin C and Lactobacillus rhamnosus in combination enhanced the growth and innate immune response of the rainbow trout, Oncorhynchus mykiss
A. Tookmehchi
atokmachi@gmail.com
1
H. Shamsi
2
S. Meshkini
3
R. Delshad
4
A. Ghasemi Moghanjoei
5
The effects of dietary vitamin C and Lactobacillus rhamnosus on immunity and growth performance were investigated in Oncorhynchus mykiss. For this purpose, 480 rainbow trout (68±5g) were obtained from a local farm and acclimated to laboratory conditions for 10 days and then divided into four groups in three replicates. During 30 days, juvenile rainbow trout were fed diets supplemented with vitamin C (1g/kg) and L. rhamnosus (at 5 × 107 CFU/g) or a control diet. Biometry was done at day -30 and blood samples were taken by caudal vein after fish anesthesia with clove powder at day 0, 15, 30 and 45. Serum lysozyme activity, alternative complement activity and total plasma immunoglobulin level were assayed as innate immune response of rainbow trout. Results showed fish fed with vitamin c and L. rhamnosus (group 3) statistically could improve fish growth performance. Also lysozyme activity and alternative complement activity of serum significantly were higher in group three than other groups, but total plasma level of immunoglobulin only was higher than all groups at day 30. In conclusion dietary administration of vitamin C and L. rhamnosus in rainbow trout diet could enhance the growth and innate immune response, but these properties need further studies on the field applications.
http://isfj.ir/article-1-723-en.pdf
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
23
30
article
Determining level of PCBs in skin and muscle tissue of Cyprinus carpio and Esox lucius in Anzali Wetland (Abkenar)
B. Teymouri
Teimouri47@yahoo.com
1
S.M.B. Nabavi
2
Sh. Safaeyan
3
S.H. Khatami
4
The aim of this research is to determine the levels of polycholoro biphenil (PCBs) in skin and muscle tissues of common carp (Cyprinus carpio) and common pike (Esox lucius) fishes in the west part of Anzali wetland (Abkenar) in the two seasons, autumn 2009 and winter 2010. In each seasons, nine fishes from each species were selected randomly. The samples, transferred in cool condition and the muscles and skins of them were removed. All of the preparations organs clean up and PCBS analysis were carried with GC-ECD (Gas chromatography). Mean concentrations of PCBS were 24.36пg/g dry.w in skin 14.36пg/g dry.w in muscle tissue for E. lucius and 21.84пg/g dry.w in skin and 20.86пg/g dry.w in muscle tissue of C. carpio. In the autumn, the values was less than FDI standard and food standard Australia/AZ MRL and skin tissue of E. lucius and muscle tissue of C. carpio was over of US EPA standard values. Mean concentration of PCBS were 29.43пg/g dry.w in skin and 10.10ng/g dry.w in muscle tissue for E. lucius and 33.06пg/g dry.w in skin and 11.00пg/g dry.w in muscle tissue for C. carpio. In the winter, the value was less than FDI standard and food standard Australia /AZ MRL and skin tissue of E. lucius and skin tissue of C. carpio was over of US EPA standard values. Mean concentration of PCBs in water in the autumn (0.0042ppb) was less than recommended water quality Criteria E.P.A and in the winter (0.1938ppb) was over recommended water quality Criteria E.P.A. Mean concentration of PCBs in sediment in the autumn (4.89ng/g dry.w) and winter (4.48ng/g dry.w) was less than Interim Canadian Sediment Quality Guidelines. There were significant differences between E. lucius mussels and C. carpio skin tissue in two seasons (autumn and winter) in water and ingredient suspension. But in sediments, no significant difference was detected in autumn and winter. *Corresponding author
http://isfj.ir/article-1-726-en.pdf
Pollution
Pollutant
Fish
Poison
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
31
44
article
Effect of freezing on the chemical quality changes and fatty acid composition of cultured shrimp muscle, Litopenaus vannamei
M. Javaheri Baboli
mehranjavaheri@gmail.com
1
R. Choi
2
A. Askary Sary
3
L. Roomiani
4
Effect of freezing on the chemical quality and fatty acid composition of cultured shrimp muscle, Litopenaus vannamei were investigated by measuring moisture content, ash, total protein content, total lipid content, fatty acid composition, and Thio barbituric Acid (TBA) during 6 month keeping in frozen storage at 18°C. According to the results, moisture content (75.93% to 73.10%), ash (1.5% to 2.07%), total protein content (25.3% to 20.87%) and total lipid content (0.83% to 0.23%) changed during six month of frozen storage. PUFA (45.21%) content was higher than the SFA (30.08%) and MUFA (19.32%) content. The poly chain unsaturated fatty acids, saturated fatty acids and mono chain unsaturated fatty acids in shrimp muscle were C18:2n6 (15.32%), C20:5n3 (9.68%), C22:6n3 (8.48%), C16:0(15.18%), C18:0 (13.04%) and C18:1n9 (15.32%), respectively. The thio barbituric acid values (TBA) ranged from 0.0065 to 0.35 mg malonaldehyde/kg during freezing storage. The lipid stability of shrimp muscle, were in acceptable limit during frozen storage for up to 6 month. *Corresponding author
http://isfj.ir/article-1-728-en.pdf
Food quality
Fisheries products
Chemical composition
Iran
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
45
54
article
A survey of salinity tolerance of Persian sturgeon larvae (Acipencer persicus) fed with enriched live food containing HUFA and vitamin C
M. Hafezieh
jhafezieh@yahoo.com
1
H. Hosseinpour
2
Salinity tolerance in Persian sturgeon larvae (Acipenser persicus) fed with enriched Artemia urmiana nauplii were investigated when exposed to different salinity. For this purpose, cyst of Artemia urmiana hatched and enriched with a commercial emulsion, ICES30/4 supplemented with three levels of vitamin C (10, 20 and 30%) each with 3 replications according to standard condition and fed to Persian sturgeon larvae during 20 experimental days. At the end of this period, total length, weight, lipid, unsaturated fatty acids contents and vitamin C in fish larvae were measured. Fish larvae were exposed to 6, 12 and 18ppt salinity, and salinity tolerance were surveyed after 120h. The results showed that survival percentage as salinity tolerance indicator increased compared to control group that are larvae fed un-enriched Artemia nauplii. ICES40/3 with 20% vitamin C led to the highest salinity tolerance at 12ppt in Persian sturgeon larvae (99±1) but in 18ppt all larvae were died. The increase in salinity tolerance is likely due to omega 3 and 6 increase in fish larvae fed with Artemia enriched with ICES30/4.
http://isfj.ir/article-1-729-en.pdf
Nutrition
Stress
Sturgeon fish
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
55
62
article
Comparison of produced fish protein hydrolysete from viscera and head of Silver carp (Hypophthalmichthys molitrix) using Alcalase enzyme and internal tissue enzymes
Sh. Hosseini
hoseini_shady@yahoo.com
1
A. Ghoroghi
2
H.R. Jamalzadeh
3
R. Safari
4
Sh. Hosseini
5
In the present study, hydrolysed protein of viscera and head of Silver carp (Hypophthalmichthys molitrix) was compared using Alcalase enzyme and internal tissue enzymes at 2 and 4 hours. The result indicated that product by Alcalase (Treatment 1) had significantly higher protein and rate of hydrolysates than that produced by internal tissue enzymes (Treatment 2). So, the highest mean (±SD) protein (68.10±1.33) was related to treatment 1-head (with Alcalase enzyme) after 4 hours and the highest rate of hydrolysates (29.36±1.35) was related to treatment 1-head (with Alcalase enzyme) after 4 hours. The result indicated that rate of hydrolysates raised as time of hydrolysates increased. However the intensity and rate of hydrolysates is reduced. The highest rate of hydrolysates occurred at 120 minutes in the first. This mode was similar for two treatments. The result can be considered as the Alcalase was preferred to internal enzyme.
http://isfj.ir/article-1-730-en.pdf
Biotechnology
Fish processing
Fish wast
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
63
72
article
Morphological study of epithelial tissue of tail spine in Cow-tail stingray (Pastinachus sephen) brown eagle ray (Aetobatus flagellum) and butterfly ray (Gymnura poecilura) in the Persian Gulf and Oman Sea
H. Dehghani
Haddehghani@gmail.com
1
M.M. Sajjadi
2
P. Parto
3
H. Rajaeian
4
J. Jalaei
5
Ray fishes have spines on their whip-like tails. In this study, histological survey was undertaken to explore venom secretory cells in spines of the 3 species of ray fish in the Persian Gulf and Oman Sea. Separated spines were preserved in formalin to transfer them to the laboratory, and spines were dip in EDTA 4%, for decalcification and in the end part, histological study were done. Results showed that venom secretory cells occurred in cow-tail stingray (P. sephen) but no venom secretory cell was found in brown eagle ray (A. flagellum) and butterfly ray (G. poecilura) species. This suggest that layers covering spine is similar to common covering structure of fishe body.
http://isfj.ir/article-1-731-en.pdf
Cartilag fish
Venom secretory cells
Persian Gulf and Oman Sea
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
73
84
article
The effects of glucan on hematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum fry
R. Rufchaie
R_rufchaie@yahoo.com
1
S.H. Hoseinifar
2
M. Sayad Borani
3
H. Maghsodie Kohan
4
A.A. Zamini
5
M. Faeed
6
The purpose of this study was to determine the effect of dietary glucan on some haematological parameters, immune response and intestinal microbiota of Rutilus frisii kutum. In the present study, various levels of ingredient so called Hoplit (0, 0.5, 1.0, 1.5, and 2.0%) containing glucan was added to a basal formulated diet. Twenty and five kutum fry with a mean (±SD) weight of 1.15±0.06g were stocked in each experimental tank (100 l capacity) filled with 80 liter of water. Fish were fed on experimental diet for 56 days and biometry was performed every 15 days. At the end of the trial blood samples were collected for measurement of haematological parameters including: Red and white blood cells count, differential count of white blood cells, hematocrit and hemoglobin, and innate immune factors (Immunoglobulin and Lysozyme). Fries in 1.5% treatment had highest serum immunoglobins (Ig) and eosinophil, although when compared with control but with no significant differences. Highest MCHC, hematocrit and hemoglobin were observed in the 0.5 and 1% treatments and the highest MCV and red blood cell count were in 0.5 percent treatment. The highest white blood cells count and neutrophils was observed in 2% treatment. The highest and lowest levels of lysozyme activity were observed in 1% and control treatments, respectively. Evaluation of the total bacteria and LAB counts revealed significant increase in 0.5% treatment. According to these results administration of dietary glucan can be considered for stimulation of innate immune response of white fish fry.
http://isfj.ir/article-1-732-en.pdf
Heamatological parameters
Fish physiology
Nutrition
Iran
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
85
98
article
Population dynamics of dominant shrimps in northeast of Qeshm Island
M. Safaie
Msn_safaie@yahoo.com
1
The Chaoo Sharghi is located nearby mangrove forest in northwest of Qeshm Island, in the northern Persian Gulf. Given standing in migration of juvenile shrimps, this area is one of important regions in Hormozgan Province. This study aimed to determine population dynamics of major shrimp stocks by swept area method in this area. Sampling was carried out monthly since March 2009 to June 2010. The average of carapace length frequency of Banana, Jinga, Kiddy and Peregrine shrimps showed that upswing from January to April. However, shrimps were predominantly juvenile. The annual growth coefficient (k) of female and male was 1.5 and 1.1 for F. merguiensis, 1.3 and 1.2 for M. affinis, 1.1 and 1 for M. stebbingi and 1.2 and 1.1 for P. stylifera, respectively. The asymptotic carapace length (CL∞) of female and male was 44mm and 31mm for P. merguiensis, 36mm and 31mm for M. affinis, 28mm and 18.5mm for M. stebbingi and 27.5mm and 18.5mm for P. stylifera, respectively. The mortality parameters of male and female shrimps were different in range including 2.36 – 4.54 for total mortality, about, 2.12–1.83 for natural mortality and 0.24–2.50 for fishing mortality. Total mortality rate for M. affinis and M. stebbingi showed that this rate for males was higher than females. Conversely, total mortality rate for F. merguiensis and P. stylifera was higher for females than males. Exploitation rate for all species was higher in females than that for males. In this study, the highest estimated biomass was found in Kiddy and Jinga shrimps and the lowest biomass was found in Indian white shrimp. The peak of biomass and CPUE was obtained during July-August.
http://isfj.ir/article-1-733-en.pdf
F. merguiensis
M. affinis
M. stebbigi
P. stylifera
Biomass
Growth and mortality Parameters
Persian Gulf
Iran
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
99
106
article
The comparison of heavy metals (Hg, Cd, Pb) in the muscle of Otolithes ruber in Abadan and Bandar Abbas Ports, the Persian Gulf
A. Askary Sary
1
M. Javahery Baboli
2
S. Mahjob
3
M. Velayatzadeh
mv.5908@gmail.com
4
A comparative study was conducted on concentration of heavy metals including: Hg, Cd and Pb in the muscle of Otolithes ruber in Abadan and Bandar Abbas Ports in the Persian Gulf. In this study, a total of 42 specimens of Otolithes ruber were collected from coastal waters of Abadan and Bandar Abbas Ports in 2010. Heavy metals were extracted from the muscle tissues using wet digestion method and concentration of metals were measured by Atomic Absorption Spectrophotometer. The results showed that mean (±SD) accumulation of Hg, Cd and Pb in the muscles of Otolithes ruber in Bandar Abbas were 0.058±0.001, 0.279±0.056 and 0.668±0.077mgKg-1 wet weight, and in Abadan were 0.056±0.007, 0.25±0.038 and 0.638±0.087mgKg-1 wet weight respectively. No significant differences were found in concentrations of Hg, Cd and Pb in the muscle of Otolithes ruber between Abadan and Bandar Abbas ports. Accumulation of these metals were higher in the muscle of Otolithes ruber than that Bandar Abbas Port. Concentration of Hg in the muscle of Otolithes ruber was lower than acceptable limit suggested by WHO, MAFF, NHMRC. However concentrations of Pb and Cd were higher. *Corresponding author
http://isfj.ir/article-1-749-en.pdf
Heavy metals
Environmental pollution
Iran
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
107
118
article
Age structure and growth rate of (Squalius cephalus Linnaeus, 1758) in Tuji tributary from Talar River, Mazandaran Province
M.H. Gorjian Arabi
hosein0037@gmail.com
1
S.A. Hosseini
2
M. Roohi
3
R. Patimar
4
S. Vatandust
5
E. Alijanpour
6
A structure and growth pattern of Squalius cephalus (Linnaeus, 1758) in Tuji tributary of Talar River, Mazandaran Province, was studied during the period from August 2008 till August 2009. In total, 298 fish were caught by electrofishing and fixed in 10% formalin. The weight and length of fish were measured and the age was determinated by scallometric method. The age data showed that fishes were between 0+ to 4+ age groups. Pauli`s applied growth pattern showed a positive alometric for male and female and total fish and a negative isometric for unmature fishes. The average instantaneous growth rate of European Chub showed that the age groups 2+ and 3+ with 1.1 had the fastest growth compare to 3+ and 4+ age groups with 0.89. Condition factor of specimens showed that the highest one with 1.58 (r2 = 0.983) belong to females and the lowest with 1.17 (r2 = 0.901) belong to unmature fishes. The total sex ratio (123 males to 115 females) showed no significant differences between them.
http://isfj.ir/article-1-746-en.pdf
Fish biology
Freshwater fish
Tuji tributary
Talar River
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
119
128
article
Changes of hormons (T3, T4 and cortisol) and ions (Na+, Cl-, K+) during smoltification in Salmo trutta caspius Kessler 1877
D. Mohammadrezaei
mrezaei@iauh.ac.ir
1
B. Majazi Amiri
2
M. Farhangi
3
Salmo trutta caspius is an important and economic fish in the Caspian Sea has several morphological and physiological changes during smoltification. In this study, Hormonal (T3, T4 & Cortisol) and ionic (Na+, Cl- & K+) changes in the serum were determined during the period of smoltification in 5, 10, 15 & 20g of hatchery reared salmo trutta caspius in different seasons (spring, summer, autumn & winter). Hormones were measured by Eliza and Radio Immuno Assay, Na+ and K+ using flame photometer, Cl- by colorimeter. T3 and T4 were quite high in spring, especially in the juvenile of 20g. Cortisol was quite low in spring and summer in all of weight groups. Ionic changes showed no significant differences with weight, but it was significantly different among seasons. This result suggests that analyzing the plasma thyroid hormones and ionic provide useful information about the optimal time of transferring Caspian Sea trout from fresh water to sea water. It is concluded that the juvenile fish of 20g shows a better smoltification process in the spring.
http://isfj.ir/article-1-747-en.pdf
Salmonidae
Fish physiology
Blood electrolytes
Caspian Sea
Iran
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
129
136
article
Evaluation of some biofilter substrates in freshwater recirculation system
A. Nekuiefard
dr.alinekuiefard@iran.ir
1
R. Manaffar
2
A.A. Motallebi Moghanjooei
3
M. Sharifian
4
The nitrifying bacteria acting on various biofilter substrates including: scoria, P.V.C., marble and zeolite were evaluated. The experiment started with manufacture of a recirculation system with 130 liter fresh water volume in 7 l.min-1 flow rate in 3 replicate for every treatment. The volume of each biofilter was 60×40×30cm and after achievement to stable environmental conditions the nitrifying bacteria at a concentration of 105ml-1were inoculated in each biofilters. Daily 3-10mg.l-1 of NH4Cl was added to biofilters. The procedure was conducted by exposure of Ammonium and Nitrate concentrations and pH in biofilters. The results showed that the Scoria can be reliable substrate as biofilter, in which provide the best substrate for the growth and attachment of the nitrifying bacteria which can reduce the retention time of a biofilter from 40-60 to 15 days.
http://isfj.ir/article-1-835-en.pdf
Ammonia
Biofiler
Mineral components
Nitrobacteria
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
137
144
article
Evaluation inhibitory effect of essential oil Savory (Satureja hortensis) in food fish
L. Yazdanpanah Goharrizi
l_yazdanpanah@yahoo.com
1
A. Sepahdari
2
E. Sharifpour
3
M. Sharifrohani
4
D. Darvishi
5
Aflatoxins are a group of fungal metabolites that are produced by the growth of fungi on food. These toxins cause illness in animals and humans, and are important in economic and humans health. In this investigation, inhibitory effects of savory (Satureja hortensis L.) essential oil were evaluated on the growth of Aspergillus flavus in fish food. A gas chromatograph apparatus linked to a mass spectrometer (GC/MS) was used to identify the effective components in Satureja hortensis essential oil after extraction. Essential oils against Aspergillus flavus incubated in PDA media and antifungal properties of essential oil Satureja hortensis was investigated. About of 300g of food samples was weighted and samples were sterilized by autoclave. Fungal suspension (3cc) was spraied into the feed samples, and various concentrations of essential oils (0, 300, 400, 500, 600ppm) added to samples. The samples were incubated at temperature of (±SD) 28±2°C. After 20-40 and 60 days period, randomly, some sampled were taken from containers and the production of aflatoxin B1, B2, G1, and G2 was measured in the laboratory. This result confirms that 500ppm concentrations of oil savory have antifungal properties against Aspergillus flavus.
http://isfj.ir/article-1-836-en.pdf
Aquatic organism
Food
Medicine plants
Fungal poisons
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
145
148
article
G.mahsa94@yahoo.com
1
2
3
4
5
http://isfj.ir/article-1-739-en.html
per
IFSRI
ISFJ
1026-1354
2322-5998
2012-10
21
3
149
154
article
The new record of the spotted catfish Arius maculates (Thunberg 1792) from Persian Gulf, Iran
P. Zare
Zare_2002k@yahoo.com
1
The species Arius maculates (Thunberg 1792) (Siluriformes, Ariidae) is recorded for the first time from the muddy shores of the inter-tidal zone of Bandar Abbas, Persian Gulf, Iran in February 2011. In this study, the morphological features of Arius maculates are described. This species has previously been recorded from Gulf of Oman to Indonesia, north to Japan (locality type). This finding considerably extends our knowledge of the distribution of Arius maculates.
http://isfj.ir/article-1-740-en.pdf
Siluridae
Systematic
Marine fish