This study was carried out in three farmed rainbow trout (Oncorhynchus mykiss) of Guilan Province. Heavy metals (Iron and Lead) were measured using Atomic absorption spectrometry in food, water and fish muscles of farms. The amounts of Iron and Lead (Mean ±SD) in all water samples from studied farms were 55.29±2.71 and 2.066±0.714 µg/L, respectively. The amounts of Iron and Lead (Mean± SD) in consumed feed of studied farms were 494.51± 89.18 and 3.494± 1.462 mg/kg, respectively. Also, the amounts of Iron and lead in fish muscle of studied farms were measured as 7.715± 1.050 and 0.39970± 0.073 mg/kg, respectively. The obtained results revealed the existence of higher amounts of iron in fish muscle above Codex standard, 2001 and lower amounts of lead levels from the mentioned standard in studied farms. The obtained data showed no significant differences in water, food and muscle of fish in studied areas (p>0.05).