The aim of this study was to determine the concentration of iron and Hazard quotient (HQ)in muscle of four marine fishes, orange - spotted grouper(Epinephelus coioides),yellow fin sea bream(Acanthopagrus latus), tongue fishes (Cynoglossus arel) ,silver pomfret(Pampus argenteus) and farmed carp fish, common carp(Cyprinus carpio), grass carp(Ctenopharyngodon idella), big head carp (Aristichthys nobilis) and silver carp(Hypophthalmichthys molitrix) from Khuzestan. Farmed fish sample was prepared from the Azadegan warm water fish culture center and marine fish was prepared from fishing ports Bahrkan, Abadan and Bandar Emam. Iron concentration measured by wet digestion and atomic absorption spectrometer Perkin Elmer 4100.The results showed the average of iron level in farmed fish was 17.08± 1.21 mg/kg dry weight and in marine fish was 10.68± 3.66 mg/kg dry weight (P <0.05).The results showed that the highest iron concentrations between farmed fish muscle was on big head carp equal to 18.16mg/kg dry weight and was significantly different(P <0.05) with grass carp and the highest iron concentrations between marine fish muscle was onTongue fishequal to15.50± 0.50mg/kg dry weight and was significantly different(P <0.05). Average hazard quotient in farmed fish was HQ=0.61and in marine fish was HQ =0.38. Hazard quotientin marine fish were in tongue fish HQ=0.55, yellow fin sea breamHQ =0. 3, Orange - spotted grouper HQ= 0.26 and silver pomfret HQ =0.41 and Hazard quotient in fish were in big head HQ=0.65, common carp HQ =0.63 grass carp HQ= 0.55and silver carp HQ =0.61. Hazard quotient in all samples was less than one and so fish nutrition no risk in terms of the amount of iron in the human body.