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:: Volume 27, Issue 1 (5-2018) ::
isfj 2018, 27(1): 59-69 Back to browse issues page
Antibacterial activity of Saponin extracted from the sea cucumber (Stichopus hermanni) collected from the Persian Gulf
Z. Salari , I. Souinezad , M. Nazemi , M. Yousefzadi
Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran
Abstract:   (923 Views)

Sea cucumbers produce various bioactive compounds especially secondary metabolites including Saponin. The aim of this study was to investigate antibacterial activity of the Saponin extracted from the Persian Gulf sea cucumber (Stichopus hermanni) in some gram positive and negative bacteria. Extraction was first done by ethanol 70% and then Saponins were separated through column chromatography with silica gel. Extracted Saponins were identified through thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were investigated via tubular dilution. Two groups of steroidal and glycosides Saponins with Rf 0.9 and 0.05 were respectively identified in fractions extracted by TLC. HPTLC results for a sample of extracted Saponin confirmed the existence of Saponin in sea cucumber regarding the Rf. The gram negative bacterium Pseudomonas aeroginosa was the most resistant one to steroidal and glycosides- steroidal Saponins with MIC of 500 and 400 micg/ml, respectively. Steroidal and glycosides- steroidal Saponins showed no bactericidal effect in gram negative bacterium Pseudomonas aeroginosa. Among the gram positive bacteria, the highest MBC of 500 micg/ml belonged to glycosides- steroidal Saponin for Staphylococcus aureus. The higher MIC in gram negative bacteria approves the lower antibacterial property of the steroidal and glycosides Saponin extracted from sea cucumber Stichopus hermanni in these bacteria compared to the gram positive ones.
 

Keywords: Sea cucumber Stichopus hermanni, Steroidal Saponin, Minimum inhibitory concentration, Gram- negative bacteria
Full-Text [PDF 851 kb]   (293 Downloads)    
Type of Study: Research | Subject: بيوتكنولوژي و فرآوري هاي شيلاتي
Received: 2017/05/25 | Accepted: 2018/05/12 | Published: 2018/05/12
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Salari Z, Souinezad I, Nazemi M, Yousefzadi M. Antibacterial activity of Saponin extracted from the sea cucumber (Stichopus hermanni) collected from the Persian Gulf . isfj. 2018; 27 (1) :59-69
URL: http://isfj.ir/article-1-1806-en.html


Volume 27, Issue 1 (5-2018) Back to browse issues page

با کسب مجوز از دفتر کمیسیون بررسی نشریات علمی وزارت علوم، تحقیات و فنآوری مجله علمی شیلات بصورت آنلاین می باشد و تعداد محدودی هم به چاپ می رساند. شماره شاپای جدید آن ISSN:2322-5998 است

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