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Application of mitochondrial DNA sequences of the control region in genetic variability of Persian sturgeon (Acipenser percicus) Populations from the Caspian Sea
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Sajad Nazari1    , Mohammad Pourkazemi2 , Majidreza Khoshkholgh3 |
1- Shahid Motahary Cold-water Fishes Genetic and Breeding Research Center
2- Iranian Fisheries Sciences Research Institute (IFSRI)
3- University of Guilan |
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Abstract: (3632 Views) |
| Direct mitochondrial DNA (mtDNA) control region sequencing analysis was used to investigate population genetic structure of Persian sturgeon (Acipenser persicus) in the Caspian Sea. A total of 45 specimens were collected from different locations of the Caspian Sea. mtDNA control region was amplified using PCR. Direct sequencing was performed according to a standard method. The results showed that 12 haplotypes were observed among 45 samples in the method. The highest numbers of haplotypes were observed in the Sefidrud River in which 3 haplotypes A, B and E among them were specific for the river and were not observed in the other locations. The average haplotype diversity (h) and nucleotide diversity (π) were 0.795±0.037 and 0.0062±0.0046, for control region sequencing, respectively. The results of Fst based on kimura- 2 parameters method and analysis of molecular variance (AMOVA) demonstrated that most variations occurred among the samples, and between samples of the Sefidrud and Russia and Azerbaijan are statistically significant (P<0.0001). Therefore, three distinct populations including Sefidrud, Russia and Azerbaijan were identified. As mtDNA control region is a highly variable segment, it may be used as a potential marker for identifying populations and for determining their management and conservation units, leading to the useful application of molecular markers in investigating conservation genetics of the Persian sturgeon. |
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| Keywords: Acipenser persicus, mitochondrial DNA, genetic variation, Caspian Sea |
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Full-Text [PDF 776 kb]
(987 Downloads)
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Type of Study: Applicable |
Subject:
ژنتيك و اصلاح نژاد
Received: 2013/02/25 | Accepted: 2020/04/19 | Published: 2020/04/21
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