In recent decades the use of algae in the food and pharmaceutical industries is of great importance. The antimicrobial activity of brown alga methanol, ethyl acetate, hexane, and chloroform extracts on bacteria gram positive, gram negative, and fungi was evaluated by using nutrient broth macrodilution test. Sargassum glaucescense was collected around the coastal waters of Chabahar (Oman Sea) in Nov and Dec 2015. Six pathogenic organisms including; Enterococcus faecium ATCC 51299, Streptococcus mutans ATCC 35668, Shigella boydii ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Salmonella enteritidi PTCC, 1709, Candida albicans ATCC 10231 and Aspergillus fumigatus PTCC 5009 were investigated by the broth dilution method. Methanolic Extract for six strains showed good activity amongst eight strains. Hexane extract, after methanolic extract has good effect on antimicrobial activity against five strains. All bacteria strain in this survey has showed resistance against ethyl acetate and chloroformic extracts. All extract of S. glaucescens has good inhibition growth against two fungal strains. S. glaucescens using four various solution extract against eight different human pathogens showed an important antimicrobial and antifungal activity. However, more investigation has to be done on separation, purification and detection of the active ingredients in order to recognize their antifungal and antibacterial activity.