This study was conducted to investigate the effects of rotifers enriched with betaine and concentrated  diet  containing  betaine  on  growth  performance,  survival  and  resistance  to salinity (10 and 50ppt), temperature (42°C) and  formalin  (100ppm)  stresses  in  Indian  white prawn (Fenneropenaeus indicus) post-larvae. A complete  randomized  design  with  three replicates per treatment in nine 20-1 tanks was chosen.  Treatments  were:  Artemia  nauplii, rotifers and concentrated diet (control); Artemia nauplii, rotifers enriched with betaine and concentrated diet (treatment 1) and Artemia nauplii, rotifers enriched with  betaine  and concentrated  diet containing betaine  (0.8%) (treatment 2). The larvae were fed 6 times per day at 4h intervals. There were significant differences in growth and survival rate between the treatments containing betaine (treatment 1 and 2) and control treatment (P<0.05). Furthermore, the larvae that were fed with betaine (treatment 1 and 2) had significantly higher survival rate in stress resistance testes (P<0.05). The present study showed that betaine has positive effect on growth performance, survival rate and resistance to stresses in Indian white prawn.