The aim of this study was to evaluate encapsulation efficiency of fish oil using different combinations of xanthan and sodium alginate (core-wall ratio of 1:2). Experimental treatments were consisted of xanthan 1%, Alginate 1%, xanthan 0.75% + Alginate 0.25%, xanthan 0.5% + Alginate 0.5% and xanthan 0.25% + Alginate 0.75% of the wall material. These experimental combinations of xanthan and sodium alginate were mixed with the other wall components. Afterward, the mixture were homogenized and subsequently freeze-dried to obtain encapsulated powder. Results revealed that encapsulated fish oil with xanthan 0.75% + Alginate 0.25% had the lowest particle size, moisture content and surface oil content as compared to the others (P<0.05). In addition, it showed the highest encapsulation efficiency, wettability and water absorption index. Treatment prepared using 1% xanthan had the lowest emulsion stability with the highest particle density which was the same as that of the treatment prepared with xanthan 0.25% + alginate 0.75%. The highest water absorption index (2.09%) was observed in the treatment containing xanthan 0.5% + alginate 0.5%. Using 1% of alginate lead to the highest lightness (75.23) for encapsulated fish oil as compared to the others (P<0.05). According to the results of the present investigation, the use of xanthan 0.75% + alginate 0.25% is suggested for the production of encapsulated fish oil with optimal properties.