The effect of replacement of fish meal with AquPro on growth and enzymes activity on juvenile rainbow trout (weight: 2.38±0.3 g) were evaluated. The fish were fed with five isoproteic and isocaloric diet for six weeks. The diets contained different level of replaced fish meal with Aqupro included 5 treatments (A=0%), (B=25%), (C=50%), (D=75%) and (E=100%). Based on the results ,weight gain, relative growth rate, protein and lipid efficiency ratio in diet 50% were significantly  higher than other treatments (p<0.05), while food efficiency ratio and feed conversion ratio were shown the highest and the lowest values in diet 50%, respectively , without a significant difference than those from other treatments (p> 0.05). The highest survival rate was revealed in the diet 75%, which was not affected by the level of AquPro in the diet. The highest activity of lipase, amylase and  acid protease enzymes, respectively, from pyloric caeca, intestine and stomach were observed in diet 50%, which showed a significant difference compared to other diets (p<0.05). The findings of growth performance and lipase, amylase and acid protease activity were revealed the diet containing 50% AquPro could be appropriate for rainbow trout growth.