[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing:
..
RSS

AWT IMAGE

..
:: Volume 27, Issue 5 (1-2019) ::
isfj 2019, 27(5): 61-69 Back to browse issues page
Isolation and molecular identification of Vibrio harveyi from cultured Asian seabass in farms located south provinces of Iran
A. Ajdari1 , R. Peyghan2 , M. Ghorbanpour2 , M. Ahangarzadeh3 , M. Mirbakhsh4
1- Department of Fish Health, Faculty of Veterinary Medicine, Chamran University of Ahvaz, Ahvaz, Iran.
2- Shahid Chamran University of Ahvaz
3- South of Iran Aquaculture Research Center
4- Iranian Fisheries science research institute
Abstract:   (4802 Views)
 
Vibrio harveyi is one of the opportunistic marine environment bacteria that are considered as one of the main causes of Asian seabass vibriosis diseases in many marine cage farms in the world. The aims of this study were isolation and identification of these bacteria in cultured Asian seabass in farms, located southern provinces of IRAN (Khuzestan, Bushehr and Hormozgan). In this study, a total number of 110 Asian seabass (80, with clinical sings of disease and 30, apparently healthy fish) with different body weight ranging 50-700g were sampled. Isolation was done from internal organs (kidney, heart, spleen and liver) in common method of bacterial isolation and isolates subjected to phenotypic and molecular characteristics using duplex PCR amplification of the 16SrDNA & vhh genes respectively genus & species-specific gene. The results of PCR showed that of the 95 phenotypically identified isolates, only 65 isolates belong to the genus vibrio and 46 (70.76%) of them were identified as Vibrio harveyi. These results indicate a high present of Vibrio harveyi in cultured Asian seabass in studied farms. Results of the biochemical characteristics of the isolates confirmed with PCR, showed isolates have different reaction in several biochemical tests. Accordingly, the duplex PCR method presented in this study was introduce as an accurate and rapid method for diagnosis of V. harveyi.

Keywords: Vibrio harveyi, Asian seabass, cage culture, Vibriosis, PCR
Full-Text [PDF 646 kb]   (1545 Downloads)    
Type of Study: Research |
Received: 2018/06/1 | Accepted: 2018/12/30 | Published: 2019/01/15
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ajdari A, Peyghan R, Ghorbanpour M, Ahangarzadeh M, Mirbakhsh M. Isolation and molecular identification of Vibrio harveyi from cultured Asian seabass in farms located south provinces of Iran . isfj 2019; 27 (5) :61-69
URL: http://isfj.ir/article-1-2062-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 27, Issue 5 (1-2019) Back to browse issues page

با کسب مجوز از دفتر کمیسیون بررسی نشریات علمی وزارت علوم، تحقیات و فنآوری مجله علمی شیلات بصورت آنلاین می باشد و تعداد محدودی هم به چاپ می رساند. شماره شاپای جدید آن ISSN:2322-5998 است

Persian site map - English site map - Created in 0.07 seconds with 40 queries by YEKTAWEB 4710