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Isolation of Aeromonas hydrophila effective phage from wastewater and using it in order to control the bacterial growth in minced meat of common carp (Cyprinus carpio)
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Ahmad Gharekhani   1 |
| 1- Islamic Azad Univercity, Maku Branch |
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Abstract: (2057 Views) |
| Bacteriophages are viruses that could destroy bacteria. Currently the use of them are increased for destroy pathogenic bacteria that transfer by food staff. The goal of this study was isolate and molecular identification of effective phage on Aeromonas hydrophila from Urmia urban sewage system and inhibits the experimental contamination in minced common carp fillet. One liter sample prepared from Urmia sewage system and transferred to the laboratory. Chloroform was used in this study for phage isolation. Also the standard strains of Aeromonas hydrophila was used to antibacterial assay of isolated bacteriophage. As well as, fish sample was prepared as fresh and minced. Then minced meat was contaminated with constant concentration of Aeromonas hydrophila and different levels of effective bacteriophage. Meat samples containing negative and positive control, four levels of bacteriophage were storage in refrigerator for a week. During the storage sampling was done for bacterial count and bacteriophage titration. Results showed that effective phage on Aeromonas hydrophila isolated from Urmia sewage system that could inhibit the growth of bacteria. Also, findings showed that the levels of 10000 and 100000 PFU/gr significantly could inhibit the growth of Aeromonas hydrophila in minced common carp meat. It’s should be concluded that there is a phage effective on Aeromonas hydrophila in Urmia sewage system that suitably could use for food staff safety. |
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| Keywords: Bacteriophage, Wastewater, Common Carp, Minced Meat, Aeromonas hydrophila |
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Full-Text [PDF 514 kb]
(516 Downloads)
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Type of Study: Research |
Subject:
ميكروبيولوژي آبزيان
Received: 2020/11/13 | Accepted: 2021/11/1 | Published: 2021/11/1
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