The solution that can also provide nutrients to support the growth of aquatic organisms, increased health, resistance to stress and pathogens are using dietary probiotics, prebiotics and synbiotic in aquatic food. This study aimed to evaluate the performance of commercial and non-commercial synbiotic on hematologic and biochemical parameters of western white shrimp hemolymph was done in 4 treatments including S1 (commercial synbiotic), S2 (non-commercial synbiotic), treatment S3 (combination of commercial and non-commercial synbiotic) and control (C) (witout synbiotic). For commercial synbiotic, a mixture of a commercial Lactobacillus spp. bacteria (at a concentration of 1.5× 10⁶ per g) and mannan oligosaccharide (at a concentration of 1%), and for non-commercial synbiotic, a mixture of a non-commercial Lactobacillus spp. (Lactobacillus plantarum) and mannan oligosaccharide was used. After 2 months of feeding, some of hemolymph indexes such as the number of total haemocytes, and biochemical and immunological parameters such as glucose, total protein, phagocytosis, phenoloxidase and total antioxidant were measured. The results showed that application of the synbiotics (both commercial and non-commercial) could increase the total number of haemocyte significantly (P<0.05). Immunological indicators and biochemical factors in the shrimp fed with different synbiotics, were beter than the control group (P<0.05). According to the results can mention that application of the synbiotics including mananoligosaccharide and Lactobacillus spp. bacteria, specialy if Lactobacillus plantarum was used, has beneficial effects on Immunological indicators of white shrimp.