In present research, effect of dietary astaxanthin levels in sources of synthetic and algal on vitamins A and E content of egg rainbow trout broodstock was investigated, totally for 120 days. It was considered seven groups consisting six treatments (T₁-T₆) in two different astaxanthin sources and control (C). According to experiment design, treatments were arranged as algal astaxanthin (Haematococcus pluvialis) in the three levels of 2.67, 3.55 and 8gr/kg food (T₁, T₂, T₃); and synthetic astaxanthin in three levels of 40, 80 and 120mg/kg food (T₄, T₅, T₆). Egg vitamins A and E content in obtained eggs from all treatments during spawning season was measured. The highest (280.88± 22.51 ng) and the lowest (147.82± 12.71ng) amount of vitamin A were observed in T2 and control group, respectively. The highest (19.71± 2.92µg) and the lowest (5.27± 0.51µg) amount of vitamin E were obtained in T3 and control group, respectively. By increasing level of astaxanthin in both sources of algal and synthetic, content of vitamin E in egg increased but the effect of algal source on these indices was more perfect. In general present study show that, feeding broodstock affected on quality content of egg, It also concluded that natural astaxanthin (Haematococcus pluvialis) for the reason that contains supplementary nutritious, is extraordinary preferable than synthetic astaxanthin to improve vitamins content of egg in rainbow trout.