[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 27, Issue 3 (8-2018) ::
isfj 2018, 27(3): 153-158 Back to browse issues page
Identification of vitellogenin gene expression patterns in liver and ovary of Rutilus frisii Kutum exposed to genistein and β-sitosterol
D. Mohammadrezaei
Department of Fisheries, Faculty of Natural Resources and Environmental, Malayer University, Malayer, I.R. Iran
Abstract:   (256 Views)
The vitellogenin gene expression can be altered by some estrogenic plant compounds like Genistein and β-sitosterol. Therefore, the measurement of vitellogenin gene expression can be used as an indicator to determine their effect on reproductive performance of aquatic animals. In order to evaluate the effects of genistein and β-sitosterol on the expression of vitellogenin gene in the liver and ovary of Rutilus frisii Kutum, the fish were separately exposed to 3 levels of genistein and beta-sitosterol (500, 50 and 10 ng/L). After 21 days, the RNA extracted and expression of vitellogenin gene in both the liver and ovary was investigated by Real-time PCR. The results showed the level of vitellogenin gene expression in fish exposed to genistein was higher in liver than control and β-sitosterol treatment. This difference was not observed in the ovarian tissue. Because the main site of vitellogenin synthesis expressed liver and it was controlled by endogenous estrogen (E2), so, it seems phytoesterogenic compound such as genistein has been able to increase the relative expression of this gene in fish Exposed to 500 ng/L.
Keywords: Genistein, β-sitosterol, vitellogenin, gene expression and Rutilus frisii Kutum
Full-Text [PDF 621 kb]   (48 Downloads)    
Type of Study: Research |
Received: 2018/02/25
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohammadrezaei D. Identification of vitellogenin gene expression patterns in liver and ovary of Rutilus frisii Kutum exposed to genistein and β-sitosterol. isfj. 2018; 27 (3) :153-158
URL: http://isfj.ir/article-1-2016-en.html


Volume 27, Issue 3 (8-2018) Back to browse issues page

با کسب مجوز از دفتر کمیسیون بررسی نشریات علمی وزارت علوم، تحقیات و فنآوری مجله علمی شیلات بصورت آنلاین می باشد و تعداد محدودی هم به چاپ می رساند. شماره شاپای جدید آن ISSN:2322-5998 است

Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 3768