This study was conducted to evaluate the effect of replacing dietary fishmeal with canola meal on growth performance, digestion, indicas hematological and thyroid hormones level of Siberian sturgeon (Acipenser baerii). Five isonitrogenous (45%crude protein) and isocaloric (18.53KJg^-1) were formulated by replacing 0(control), 10 (CM10), 20(CM20), 30(CM30) and 40%(CM40) of fishmeal with canola meal. Fish juveniles with initial weight of 22.80±0.34 g (mean ± SD) in 5 treatments and 3 replications were fed for 10 weeks. At the end of the experiment, growth performance, digestion, hematological parameters and T₃ and T₄ hormone levels were measured. The results of this study showed that there were significant differences in growth indices, body composition and T₄ level of fish fed different diets (p<0.05). There were no significant differences in growth performance between control, CM10, CM20 and CM30, but the lowest growth performance were observed in CM40 and there was significant differences in growth performance between CM40 with other treatments. There were no significant difference in growth performance between CM30 and CM40. There were no significant differences in hematological indices between fish fed differences diets. There were no significant differences in T₃ level between of fish fed differences diets, but significant differences were observed in T₄ level in fish fed different diets. The lowest level of T₄ was observed in CM40 and there were significant differences with control, CM10 and CM30. There were no significant differences in T₄ level between CM20 with CM30 and CM40,but were significant differences between CM20 with control and CM10. There were no significant differences in T₃:T₄ ratio between fish fed different diets. The results of the present study showed that 30%fish meal can be replaced by canola meal without negative effect on growth performance, body composition, hematological parameters and thyroid hormone levels of Siberian sturgeon.