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:: Volume 30, Issue 4 (11-2021) ::
isfj 2021, 30(4): 1-12 Back to browse issues page
Investigation of antibacterial activity of Virgularia juncea extracts from the Persian Gulf
Reza Mohsenian Kouchaksaraee, Melika Nazemi, Mahdi Moridi Farimani *
Shahid Beheshti University
Abstract:   (225 Views)
Although Iran covers most of the northern coast of the Persian Gulf, marine organisms from this region has have not been investigated very well. Herein, we aimed at a detailed analysis of antibacterial profiles against Escherichia coli ATCC, Bacillus subtilis spizizenii ATCC and Staphylococcus aureus ATCC of the marine sea pen Virgularia juncea collected from the Persian Gulf in Iran. To isolate the active ingredients, a bio-guided purification approach was used based on the MIC and MBC parameters obtained for the extracts and fractions. The n-hexane, ethyl acetate and methanol extracts of V. juncea were tested in multiple in-house bioassays, and the ethyl acetate extract showed in vitro antibacterial activity against S. aureus (MIC value 7.5 µg/mL). This extract was fractionated by using open column chromatography. The isolated active fraction (F6d), which had high activity against the S. aureus strain at the lowest concentrations (MBC value 10 µg/mL) of other fractions, was purified using the HPLC method. The pure compound Cholest-5-en-3β-ol was isolated from the active fraction (F6d), and showed moderate activity against the S. aureus bacterial strain (MBC value 50 µg/mL). This compound was identified using the GC-MS technique. This is the first study that has dealt with bioactivity profiling of V. juncea, leading to isolation and identification of pure sea pen metabolite.
Keywords: Persian Gulf, Marine natural products, Sea pen, Virgularia juncea, Antibacterial
Full-Text [PDF 453 kb]   (89 Downloads)    
Type of Study: Research | Subject: ميكروبيولوژي آبزيان
Received: 2020/08/16 | Accepted: 2021/11/1 | Published: 2021/11/1
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Mohsenian Kouchaksaraee R, Nazemi M, Moridi Farimani M. Investigation of antibacterial activity of Virgularia juncea extracts from the Persian Gulf. isfj. 2021; 30 (4) :1-12
URL: http://isfj.ir/article-1-2437-en.html


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Volume 30, Issue 4 (11-2021) Back to browse issues page

با کسب مجوز از دفتر کمیسیون بررسی نشریات علمی وزارت علوم، تحقیات و فنآوری مجله علمی شیلات بصورت آنلاین می باشد و تعداد محدودی هم به چاپ می رساند. شماره شاپای جدید آن ISSN:2322-5998 است

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