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Effect of acidic, enzymatic and ultrasonic extraction methods on the characteristics of fucoidan from Sargassum ilicifolium
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M. Alboofetileh   |
| Fish Processing Technology Research Center, Iranian Fisheries Science Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran |
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Abstract: (550 Views) |
| In the present study, fucoidan was extracted from Sargassum ilicifolium by acidic, enzymatic, and ultrasonic methods. The extraction yield was determined and Fourier-transform infrared spectroscopy (FTIR) was used to identify the functional groups of the extracted fucoidans. Antioxidant activity of fucoidan was evaluated based on the scavenging of DPPH free radical and ferric reducing antioxidant power (FRAP) assays. Emulsifying properties of the isolated fucoidan were evaluated using sunflower, corn, and canola oils. The results showed that the extraction yield of fucoidan in the enzyme method (11.37%) was significantly higher than those obtained by acidic (7.87%) and ultrasound (8.07%) methods (p<0.05). The FTIR spectra demonstrated that the extracted fucoidans possess sulphate groups at 817 and 1249 cm-1. The highest DPPH radical scavenging (38.07%) and reducing power (absorption 0.167) activities were measured in the fucoidan extracted at 1 mg/ml by the enzymatic method (p<0.05). On the contrary, fucoidan extracted by the acidic method had the lowest DPPH radical scavenging (26.42%) and reducing power (0.156 Abs) activities (p<0.05). The highest emulsification index (34.67-39.75%) was measured in fucoidan extracted by enzymatic method. However, the emulsification index values were just significantly different in corn oil (p<0.05). Generally, the fucoidan extracted using the ultrasonic and enzymatic methods showed higher yield, antioxidant, and emulsifying properties than those extracted by the acidic method. |
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| Keywords: Extraction methods, Bioactive compounds, Sulfated polysaccharides, Bioactive properties, Functional properties |
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Full-Text [PDF 932 kb]
(273 Downloads)
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Type of Study: Research |
Subject:
بيوتكنولوژي و فرآوري هاي شيلاتي
Received: 2024/04/26 | Accepted: 2024/04/29 | Published: 2024/06/18
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