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Effect of Vista pesticide on liver tissue and some serum parameters in grass carp (Ctenopharyngodon idella)
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Seyede Zeinab Hosseini koohkheili1   , Shila Omidzahir1 , Seyyed Mohammad Hosseini2 , Abdolali Movahedinia1 |
1- Faculty of Marine and environmental Sciences, University of Mazandaran, Babolsar, Iran
2- Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran |
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Abstract: (439 Views) |
Pesticides and their residues are among the most important polluting factors of aquatic ecosystems. Vista is a combination fungicide used to control blast disease in rice. Despite the effective use of Vista fungicide in blast disease control, the environmental effects of this pesticide have not been investigated in aquatic animals. Therefore, the purpose of this research was to investigate the effects of Vista fungicide on liver tissue and some blood serum parameters in grass carp (Ctenopharyngodon idella). For this purpose, 180 grass carp were divided into 4 groups in 3 replications. Group 1 was considered as the control group, and groups 2, 3 and 4 received 5%, 10% and 20% of the 96-hour LC50 of Vista equal to 1.56, 3.128 and 6.25 mg/l for 28 days, respectively. The results of the examination of serum parameters showed that the amount of total protein and albumin decreased with the increase of Vista concentration, and a significant decrease was observed in group 4 (6.25 mg/l of Vista) compared to group 1 (control) and group 2 (1.56 mg/l of Vista) (p˂0.05). The amount of glucose, cholesterol, and triglycerides increased with the increase in the concentration of Vista, so that the highest amount was observed in group 4, which had a significant difference in the case of glucose compared to other groups (p<0.05), and in the case of cholesterol and triglycerides showed a significant difference compared to the groups 1 and 2 (p<0.05). The amounts of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) enzymes increased with increasing Vista concentration. ALP and AST enzymes had a significant increase in group 4 compared to groups 1 and 2 (p˂0.05), and ALT enzyme had a significant increase in the groups receiving Vista compared to group 1 (p˂0.05). Histological examination showed that the livers of fish in group 1 had normal conditions. The symptoms were mild hyperemia in group 2, moderate hyperemia, vacuolar degeneration of liver cells, and necrosis of liver cells with a mild degree in group 3, and severe hyperemia, vacuolar degeneration of liver cells and necrosis of liver cells with a moderate degree in group 4 were observed. The results of this research indicate that increasing the concentration of Vista leads to increase hepatic tissue damages and biochemical changes in the blood serum of studied fish. |
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| Keywords: Pesticide, Toxicology, Histopathology, Serology, Vista, Grass carp |
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Full-Text [PDF 1036 kb]
(150 Downloads)
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Type of Study: Research |
Subject:
آلودگي محيطهاي آبي
Received: 2024/07/28 | Accepted: 2024/08/31 | Published: 2024/10/2
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